Determination of cyclooxygenase products and prostaglandin metabolites using high-pressure liquid chromatography and radioimmunoassay. [Tritium tracer technique]
Journal Article
·
· Anal. Biochem.; (United States)
A method is described for measurement for the cyclooxygenase products, thromboxane, prostacyclin, and prostaglandins (PG), and several prostaglandin metabolites. The procedure involves separation of the compounds by high-pressure liquid chromatography combined with identification and estimation by serologic analysis. These combined procedures have been used to identify and estimate five such products, PGE/sub 2/, PGE/sub 1/, PGF/sub 2..cap alpha../, PGF/sub 1..cap alpha../, and 6-keto-PGF/sub 1..cap alpha../ in the culture fluids of dog kidney cells stimulated by a tumor-promoting phorbol diester. The prostaglandin metabolites, 13,14-dihydro-15-keto-PGE/sub 2/, 13,14-dihydro-15-keto-PGF/sub 2..cap alpha../, 13,14-dihydro-PGE/sub 2/, and 13,14-dihydro-PGF/sub 2..cap alpha../, were not found in these culture fluids.
- Research Organization:
- Brandeis Univ., Waltham, MA
- OSTI ID:
- 6333807
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 93:2; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHROMATOGRAPHY
DATA
DATA FORMS
ENZYMES
EXPERIMENTAL DATA
GRAPHS
HYDROGEN ISOTOPES
INFORMATION
ISOLATED VALUES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
METABOLITES
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
OXIDASES
OXIDOREDUCTASES
PROSTAGLANDINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM
YEARS LIVING RADIOISOTOPES
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHROMATOGRAPHY
DATA
DATA FORMS
ENZYMES
EXPERIMENTAL DATA
GRAPHS
HYDROGEN ISOTOPES
INFORMATION
ISOLATED VALUES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
METABOLITES
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
OXIDASES
OXIDOREDUCTASES
PROSTAGLANDINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM
YEARS LIVING RADIOISOTOPES