Tritium labeled amino acid conjugates of prostaglandins and thromboxanes as labeled ligands in prostanoid radioimmunoassay

Journal Article · Sun Sep 01 04:00:00 EDT 1985 · Prostaglandins; (United States)

DOI:https://doi.org/10.1016/0090-6980(85)90117-0· OSTI ID:6234352

Sautebin, L; Kindahl, H; Kumlin, M; Granstroem, E

Conjugates of prostaglandins and thromboxanes with tritium labeled amino acids were prepared and employed as labeled ligands in prostaglandin and thromboxane radioimmunoassays. Assays for PGF2 alpha, 15-keto-13, 14-dihydro-PGF2 alpha, TXB2 and 15-keto-13,14-dihydro-TXB2 were evaluated in comparative studies using either these heterologous ligands or the corresponding homologous tritiated eicosanoid as tracers. Binding properties for the respective antibodies were found to be similar using either tracer. Three biological studies were also conducted, viz. study of the release of TXB2 during collagen induced platelet aggregation, of 15-keto-13,14-dihydro-TXB2 during guinea pig pulmonary anaphylaxis, and of PGF2 alpha during bovine luteolysis. The analyses gave comparable results using either the heterologous or the homologous assay. Thus, this type of labeled prostanoid conjugates may serve as a convenient alternative to homologous tracers in radioimmunoassay. Heterologous tracers may even in certain cases provide the only simple solution to the problem of preparing a labeled ligand of high specific activity.

• Research Organization: Karolinska Institutet, Stockholm, Sweden
• OSTI ID: 6234352
• Journal Information: Prostaglandins; (United States), Journal Name: Prostaglandins; (United States) Vol. 3; ISSN PRGLB
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIGANDS
LIQUID COLUMN CHROMATOGRAPHY
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROSTAGLANDINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS