Stimulation of arachidonic acid metabolism in primary cultures of osteoblast-like cells by hormones and drugs

Journal Article · Sat Dec 01 04:00:00 EST 1984 · Prostaglandins; (United States)

DOI:https://doi.org/10.1016/0090-6980(84)90034-0· OSTI ID:5655793

Feyen, J H; van der Wilt, G; Moonen, P; Di Bon, A; Nijweide, P J

The effects of parathyroid hormone (PTH), dihydroxycholecalciferol (1,25-(OH)2 D3), thrombin, epidermal growth factor (EGF) and 12-o-tetradecanoylphorbol-13-acetate (PMA) on the biosynthesis and release of arachidonic acid metabolites were studied in primary cultures of osteoblast-like cells isolated from 18-day-old chick embryo calvaria. Cells were labelled with (/sup 14/C)-arachidonic acid for 30 h. The radioactive eicosanoids were extracted from the cell culture media after a further 30 h stimulation period and analysed on a PRP-1 column by HPLC. The radioactive products were characterized by co-elution of (/sup 3/H) standard prostanoids. Osteoblasts showed a basal release of the prostanoids 6-keto-PGF1 alpha, TXB2, PGF2 alpha, PGE2, PGD2 and PGB2, the latter being the most abundant one. Indomethacin (10(-5) M) effectively inhibited the basal release, but not that of an as yet unidentified compound. The release of prostanoids was stimulated by PTH (2 U/ml), thrombin (0.4 NIH/ml), EGF (50 ng/ml) and PMA (25 ng/ml), the latter being by far the most potent one. 1,25-(OH)2D3 was found to slightly inhibit the prostanoid release. These results indicate: (1) primary cultures of osteoblasts synthesize several prostaglandins, thromboxane B2 and one unidentified product. (2) the action on bone of PTH and the various drugs tested may be, at least partly, mediated by an increased prostaglandin production by osteoblasts. Clearly this does not apply to 1,25-(OH)2D3.

• Research Organization: Univ. of Leiden, The Netherlands
• OSTI ID: 5655793
• Journal Information: Prostaglandins; (United States), Journal Name: Prostaglandins; (United States) Vol. 28:6; ISSN PRGLB
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARACHIDONIC ACID
BIOSYNTHESIS
BIRDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CULTURES
CHICKENS
CHOLECALCIFEROL
CHROMATOGRAPHY
COAGULANTS
DRUGS
EMBRYOS
ENZYMES
FOWL
HEMATOLOGIC AGENTS
HEMOSTATICS
HORMONES
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
METABOLISM
METABOLITES
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARATHORMONE
PEPTIDE HORMONES
PEPTIDE HYDROLASES
PROSTAGLANDINS
RESPONSE MODIFYING FACTORS
SEPARATION PROCESSES
SERINE PROTEINASES
SYNTHESIS
THROMBIN
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
VITAMIN D
VITAMINS