Subcellular distribution of curium in beagle liver

Journal Article · Tue Jun 01 04:00:00 EDT 1976 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574450· OSTI ID:7175812

Bruenger, F W; Grube, B J; Atherton, D R; Taylor, G N; Stevens, W

The subcellular distribution of curium (/sup 243/ /sup 244/Cm) was studied in canine liver from 2 hr to 47 days after injection of 3 ..mu..Ci/sup 243/ /sup 244/Cm/kg of body weight. The pattern of distribution for Cm was similar to other trivalent actinide elements studied previously (Am, Cf). Initially (2 hr), most of the nuclide was found in the cytosol and at least 90 percent was protein bound. About 70 percent of the Cm was bound to ferritin, approximately 5 percent was associated with a protein of MW approximately 200,000, and approximately 25 percent was found in the low-molecular-weight region (approximately 5000). The decrease in the Cm content of cytosol, nuclei, and microsomes coincided with an increase in the amount associated with mitochondria and lysosomes. The concentration of the Cm in the mitochondrial fraction was higher than it was in the lysosomal fraction at each time studied. In the mitochondrial fraction approximately 30 percent of the Cm was bound to membranous or granular material, and 70 percent was found in the soluble fraction. The Cm concentration initially associated with cell nuclei was high but had diminished to 20 percent of the 2 hr concentration by 20 days post injection (PI). The subcellular distribution of Cm in the liver of a dog which had received the same dose and was terminated because of severe liver damage was studied at 384 days PI. The liver weighed 130 g and contained approximately 30 percent of the injected Cm. In contrast, a normal liver weighs 280 g and at 2 hr PI contains approximately 40 percent of the injected dose. The subcellular distribution of Cm in this severely damaged liver differed from the pattern observed at earlier times after injection. The relative concentration of Cm in the cytosol was doubled; it was higher in the nuclei-debris fraction; and it was lower in the mitochondrial and lysosomal fractions when compared to earlier times.

• Research Organization: Univ. of Utah, Salt Lake City
• OSTI ID: 7175812
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 66:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560172* -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACTINIDE ISOTOPES
ACTINIDE NUCLEI
ALPHA DECAY RADIOISOTOPES
ANIMALS
BEAGLES
BODY
CELL CONSTITUENTS
CELL NUCLEI
CURIUM 243
CURIUM 244
CURIUM ISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
DOGS
EVEN-EVEN NUCLEI
EVEN-ODD NUCLEI
GLANDS
HEAVY NUCLEI
INJECTION
INTAKE
INTRAVENOUS INJECTION
ISOTOPES
LIVER
LYSOSOMES
MAMMALS
MICROSOMES
MITOCHONDRIA
NUCLEI
ORGANOIDS
ORGANS
RADIOISOTOPES
RADIONUCLIDE KINETICS
TIME DEPENDENCE
VERTEBRATES
YEARS LIVING RADIOISOTOPES