Subcellular distribution of styrene oxide in rat liver

Journal Article · Sun Jan 01 04:00:00 EST 1984 · Toxicol. Pathol.; (United States)

DOI:https://doi.org/10.1177/019262338401200111· OSTI ID:5765768

Pacifici, G M; Cuoci, L; Rane, A

The subcellular distribution of (/sup 3/H)-styrene-7,8-oxide was studied in the rat liver. The compound was added to liver homogenate to give a final concentration of 2 X 10(-5); 2 X 10(-4) and 2 X 10(-3) M. Subcellular fractions were obtained by differential centrifugation. Most of styrene oxide (59-88%) was associated with the cytosolic fraction. Less than 15 percent of the compound was retrieved in each of the nuclear, mitochondrial and microsomal fractions. A considerable percentage of radioactivity was found unextractable with the organic solvents, suggesting that styrene oxide reacted with the endogenous compounds. The intracellular distribution of this epoxide was also studied in the perfused rat liver. Comparable results with those previously described were obtained. The binding of styrene oxide to the cytosolic protein was investigated by equilibrium dialysis and ultrafiltration. Only a small percentage of the compound was bound to protein.

• Research Organization: Univ. of Pisa, Italy
• OSTI ID: 5765768
• Journal Information: Toxicol. Pathol.; (United States), Journal Name: Toxicol. Pathol.; (United States) Vol. 12:1; ISSN TOPAD
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKYLATED AROMATICS
ANIMALS
AROMATICS
BODY
CELL CONSTITUENTS
CENTRIFUGATION
CHALCOGENIDES
CHEMICAL BONDS
DIGESTIVE SYSTEM
DISTRIBUTION
EPOXIDES
GLANDS
HYDROCARBONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIVER
MAMMALS
MICROSOMES
MITOCHONDRIA
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANIC SOLVENTS
ORGANOIDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PERFUSED ORGANS
PROTEINS
RATS
RODENTS
SEPARATION PROCESSES
SOLVENTS
STYRENE
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES