Chemical form of cadmium in subcellular fractions following cadmium exposure

Journal Article · Thu Mar 01 04:00:00 EST 1984 · Environ. Health Perspect.; (United States)

DOI:https://doi.org/10.1289/ehp.845437· OSTI ID:5853211

Waku, K

Wistar rats were given drinking water containing 250 ppm Cd for 12 months. After excision of the kidney and liver, the organs were subfractionated into nuclear, mitochondrial, microsomal and cytosol fractions, and the chemical forms of Cd in the subcellular fractions were examined. Although approximately 90% of the total Cd was present in the cytosol, in the form of metallothionein, 3-5% was also present in the mitochondrial fraction and 5-7% in the microsomal fraction from both organs. By Sephadex G-75 gel filtration, after solubilizing the particulate fractions with sodium deoxycholate, approximately 89% of Cd in the microsomal fraction and 94% in the mitochondrial fraction eluted with the same retention time as that of metallothionein in both liver and kidney, while the remainder was found in a high molecular weight protein fraction. The Cd that eluted with the high molecular protein fraction may be involved in dysfunction in subcellular organelles. For estimating the toxicity of Cd associated with the high molecular weight fraction, rat liver microsomes and CdCl/sub 2/ were mixed with 1% of sodium deoxycholate and the protein-Cd complex produced was isolated by eluting with Sephadex G-75. This complex had a strong toxicity toward the alcohol dehydrogenase activity (SH-enzyme), and the K/sub i/ values of the Cd-protein complexes decreased with increased amount of Cd bound to the microsomal protein fraction. The above results suggest that loosely bound Cd increased in the case of higher Cd/protein and plays a toxic role in the living cells. 25 references, 6 figures, 3 tables.

• Research Organization: Teikyo Univ., Sagamiko, Japan
• OSTI ID: 5853211
• Journal Information: Environ. Health Perspect.; (United States), Journal Name: Environ. Health Perspect.; (United States) Vol. 54; ISSN EVHPA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BODY
CADMIUM COMPLEXES
CELL CONSTITUENTS
CHEMICAL BONDS
CHRONIC EXPOSURE
COMPLEXES
DATA
DIGESTIVE SYSTEM
DRINKING WATER
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
GLANDS
HYDROGEN COMPOUNDS
INFORMATION
INHIBITION
KIDNEYS
LIVER
MAMMALS
METALLOPROTEINS
METALLOTHIONEIN
MICROSOMES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PROTEINS
RATS
RODENTS
STRUCTURE-ACTIVITY RELATIONSHIPS
TOXICITY
VERTEBRATES
WATER