Interactions of gold with cytosolic selenium-containing proteins in rat kidney and liver

Journal Article · Fri Apr 01 04:00:00 EST 1988 · J. Inorg. Biochem.; (United States)

DOI:https://doi.org/10.1016/0162-0134(88)85008-6· OSTI ID:6855870

Hu, M L; Viljoen, A J; Tappel, A L

Rats injected with aurothioglucose (ATG) for 5 days were subsequently injected with (/sup 75/Se)selenious acid and killed after 3 days. Kidney and liver cytosols were chromatographed on Sephadex G-150. /sup 75/Se in kidney was associated with high molecular weight (HMW), 85,000 Mr, 26,000 Mr, and 10,000 Mr proteins and with a nonprotein fraction. The elution profile of liver cytosol was similar to that of kidney, but without a 26,000 Mr protein. ATG injection increased the association of /sup 75/Se with all fractions of kidney cytosol except the 85,000 Mr fractions, which contained Se-glutathione peroxidase (SeGSHPx) activity; /sup 75/Se in liver was increased only in HMW fractions. Unfractionated kidney cytosolic SeGSHPx activity was decreased 14% by ATG injection, but liver enzyme activity was not changed. However, Sephadex G-150 chromatography showed that total and specific activities, respectively, were decreased 28 and 23% in kidney and 25 and 16% in liver. Au coeluted with HMW and 10,000 Mr /sup 75/3Se-containing kidney proteins; the latter contained 50% of the Au eluted from the column. DEAE Sephacel chromatography of the 10,000 Mr kidney protein showed that both Au and /sup 75/Se were tightly associated with metallothionein-like proteins. This study demonstrates the interaction of Au with rat liver and kidney /sup 75/Se-containing proteins.

• Research Organization: Univ. of California, Davis (USA)
• OSTI ID: 6855870
• Journal Information: J. Inorg. Biochem.; (United States), Journal Name: J. Inorg. Biochem.; (United States) Vol. 32:4; ISSN JIBID
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
GLANDS
GOLD
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
KINETICS
LIVER
MAMMALS
METABOLISM
METALLOPROTEINS
METALLOTHIONEIN
METALS
MOLECULAR WEIGHT
NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEROXIDASES
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RODENTS
SELENIUM
SELENIUM 75
SELENIUM ISOTOPES
SEMIMETALS
SEPARATION PROCESSES
TRACER TECHNIQUES
TRANSITION ELEMENTS
VERTEBRATES