Chemical forms of selenium in rat tissues after administration of selenite or selenomethionine
The chemical forms of selenium (Se) were determined in erythrocyte and liver proteins after injection of /sup 75/Se as either sodium selenite or selenomethionine (Se-Met) in male weanling rats. Gel-filtration chromatography (Sephadex G-150) of erythrocyte lysate revealed labeling of four fractions corresponding to void volume proteins, glutathione peroxidase (GPx), hemoglobin (Hb) and low-molecular-weight materials. Acid hydrolysates of erythrocyte protein fractions and whole liver were analyzed by ion-exchange chromatography (Dionex DC6A). Void volume proteins contained principally selenocysteine (/sup 75/Se-Cys) in (/sup 75/Se)selenite-injected animals. This material contained both /sup 75/Se-Met and /sup 75/Se-Cys 1 d postinjection in /sup 75/Se-Met-injected animals, but primarily /sup 75/Se-Cys at 20 d afterwards. GPx contained /sup 75/Se as /sup 75/Se-Cys regardless of the selenium compound injected. Hb of /sup 75/Se-Met-injected animals contained principally /sup 75/Se-Met at both 1 and 20 d postinjection. In (/sup 75/Se)selenite-injected animals, /sup 75/Se was present in hemoglobin as two unidentified forms. In acid hydrolysates of whole liver /sup 75/Se was recovered principally as /sup 75/Se-Cys from animals injected with (/sup 75/Se)selenite. For animals injected with /sup 75/Se-Met, liver /sup 75/Se was present initially as /sup 75/Se-Met, but after 5 d the majority of liver /sup 75/Se was as Se-Cys. No differences were found in deposition of /sup 75/Se in liver, kidney, testes, erythrocytes or plasma in rats injected with labeled selenite or Se-Met, but a significantly greater retention was found in muscle of Se-Met-injected rats as compared to those given selenite.
- Research Organization:
- Oregon State Univ., Corvallis
- OSTI ID:
- 6933568
- Journal Information:
- J. Nutr.; (United States), Journal Name: J. Nutr.; (United States) Vol. 9; ISSN JONUA
- Country of Publication:
- United States
- Language:
- English
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560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CHEMISTRY
CYSTEINE
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ERYTHROCYTES
EVEN-ODD NUCLEI
GLANDS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIVER
MAMMALS
MATERIALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
RADIOISOTOPES
RATS
RODENTS
SELENIUM
SELENIUM 75
SELENIUM ISOTOPES
SEMIMETALS
THIOLS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
VERTEBRATES