Selenium-containing proteins of rat kidney

Journal Article · Mon Aug 01 04:00:00 EDT 1988 · J. Inorg. Biochem.; (United States)

DOI:https://doi.org/10.1016/0162-0134(88)80005-9· OSTI ID:6540003

Viljoen, A J; Tappel, A L

Rat kidney selenium (Se)-containing proteins were studied by isotopic labeling with (/sup 75/Se)selenite or (/sup 75/Se)selenomethionine via three routes: oral, intraperitoneal injection, and incubation of kidney slices with the isotope. The two major Se-containing proteins in kidney were fractionated and partially characterized. /sup 75/Se elution profiles from Sephadex G-150 chromatography were similar for each labeling protocol, except for the profile obtained following incubation of slices with (/sup 75/Se)selenomethionine. Of the two major /sup 75/Se-containing proteins, the one eluting at the void volume during Sephadex G-150 fractionation had a subunit of 23,000 Mr. The /sup 75/Se-labeled tryptic peptide from this protein and a /sup 75/Se-containing tryptic peptide from glutathione peroxidase had the same elution time from an HPLC column. A 75,000 Mr /sup 75/Se-containing protein had a 65,000 Mr subunit, and the /sup 75/Se-labeled tryptic peptide from this protein eluted from the HPLC column before that of glutathione peroxidase. Glutathione peroxidase is the most abundant kidney selenoprotein. Injection of animals with /sup 75/Se is the method of choice for isotopic labeling of rat kidney Se-containing proteins. Appropriate methods were developed that can be used in future studies of kidney Se-containing proteins.

• Research Organization: Univ. of California, Davis (USA)
• OSTI ID: 6540003
• Journal Information: J. Inorg. Biochem.; (United States), Journal Name: J. Inorg. Biochem.; (United States) Vol. 33:4; ISSN JIBID
• Country of Publication: United States
• Language: English

Related Subjects

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560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ENZYMES
EVEN-ODD NUCLEI
FRACTIONATION
HYDROLASES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LIPOTROPIC FACTORS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
METABOLISM
METHIONINE
MOLECULAR WEIGHT
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEPTIDE HYDROLASES
PEROXIDASES
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SELENIUM
SELENIUM 75
SELENIUM ISOTOPES
SEMIMETALS
SEPARATION PROCESSES
SERINE PROTEINASES
TRACER TECHNIQUES
TRYPSIN
VERTEBRATES