Separation and characterization of rat kidney and liver selenium-containing proteins

Thesis/Dissertation · Fri Jan 01 04:00:00 EST 1988

OSTI ID:7003164

Viljoen, A J

Selenium (Se)-containing proteins in rat kidney cytosol were studied after ({sup 75}Se)selenite or ({sup 75}Se)selenomethionine labeling via three routes: oral, intraperitoneal injection, and incubation of kidney slices with isotope. {sup 75}Se gel-filtration chromatography elution profiles were similar for each protocol, except after slice incubation with ({sup 75}Se)selenomethionine. The major {sup 75}Se-containing cytosolic kidney proteins were glutathione peroxidase (SeGSHPx) and a 75,000Mr protein with a 65 kDa {sup 75}Se-containing subunit. A {sup 75}Se-containing tryptic peptide from this protein eluted from a HPLC reverse-phase column before a similar SeGSHPx peptide. The interaction of metallothionine-like proteins (MTLP), Se, and Cd was studied after rats were injected with ({sup 75}Se)selenite and CdCl{sub 2}. Se-containing proteins in ({sup 75}Se)selenite-labeled rat kidney and liver microsomes were investigated.

• Research Organization: California Univ., Davis, CA (USA)
• OSTI ID: 7003164
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CADMIUM
CADMIUM CHLORIDES
CADMIUM COMPOUNDS
CADMIUM HALIDES
CELL CONSTITUENTS
CHEMICAL COMPOSITION
CHLORIDES
CHLORINE COMPOUNDS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ENZYMES
EVEN-ODD NUCLEI
FRACTIONATION
GEL PERMEATION CHROMATOGRAPHY
GLANDS
HALIDES
HALOGEN COMPOUNDS
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTRAPERITONEAL INJECTION
ISOTOPES
KIDNEYS
KINETICS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
MAMMALS
METABOLISM
METALLOPROTEINS
METALS
MICROSOMES
NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
PEROXIDASES
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RIBOSOMES
RODENTS
SELENIUM
SELENIUM 75
SELENIUM ISOTOPES
SEMIMETALS
SEPARATION PROCESSES
VERTEBRATES