Purification and characterization of rat plasma selenoprotein P

Thesis/Dissertation · Sun Jan 01 04:00:00 EST 1989

OSTI ID:6787513

Motchnik, P A

Selenoprotein P was fractionated and characterized from {sup 75}Se-labeled plasma of rats and characterized. Using salt precipitation, affinity gel blue and diethylaminoethyl ion exchange column chromatography, selenoprotein P was 225-fold purified. The molecular weight of selenoprotein P was 98,000 and the pI was 5.4. The {sup 75}Se-containing subunit of selenoprotein P was purified to 90% homogeneity using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by electroelution. This subunit had an apparent molecular weight of 57 kDa. Reversed-phase HPLC and Sephadex G-50 chromatography of tryptic peptides of the subunit yielded several peaks of {sup 75}Se radioactivity, indicating that {sup 75}Se is present in several locations within the 57 kDa subunit of selenoprotein P. The distribution of {sup 75}Se 3, 12, and 48 h post injection of {sup 75}SeO{sub 3}{sup 2{minus}} was studied in the plasma, and homogenates and microsomes of kidney and liver of selenium-deficient and selenium-supplemented rats. There was a concurrent increase in the labeling of lower molecular weight subunits. The significance of the time-dependent {sup 75}Se distribution in the subunits is discussed.

• Research Organization: California Univ., Davis, CA (USA)
• OSTI ID: 6787513
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BETA DECAY RADIOISOTOPES
CHEMICAL COMPOSITION
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
FRACTIONATION
INTERMEDIATE MASS NUCLEI
ION EXCHANGE CHROMATOGRAPHY
ISOTOPE APPLICATIONS
ISOTOPES
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
METABOLISM
METALLOPROTEINS
MOLECULAR WEIGHT
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SELENIUM
SELENIUM 75
SELENIUM ISOTOPES
SEMIMETALS
SEPARATION PROCESSES
TIME DEPENDENCE
TISSUE DISTRIBUTION
TRACER TECHNIQUES
VERTEBRATES