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Title: Isolation and properties of cadmium-binding protein induced in rat small intestine by oral administration of cadmium

Abstract

The chromatographic properties and amino acid composition of Cd-binding protein induced in the rat small intestine by oral administration of Cd were examined. When the intestinal mucosal cytosol from rats treated orally with Cd was chromatographed on Sephadex G-75, Cd in the intestinal mucosa was bound 95.5% to protein of molecular weight about 10,000. A DEAE Sephadex A025 chromatogram of the Cd-binding protein from Sephadex G-75 showed two major and one minor Cd-containing peaks as hepatic and renal metallothionein. The amino acid composition of the two major Cd peaks resembled that of metallothionein from other materials examined. These results indicate that the low-molecular-weight Cd-binding proteins in the small intestinal mucosa meet the criteria for classification as metallothionein, and suggest that induced intestinal Cd-binding protein may be capable of preventing the transport of orally administered Cd from the intestinal mucosa into the other organs.

Authors:
;
Publication Date:
Research Org.:
Kochi Medical School, Nankoku-Shi, Kochi-Ken, Japan
OSTI Identifier:
5299835
Resource Type:
Journal Article
Journal Name:
J. Toxicol. Environ. Health; (United States)
Additional Journal Information:
Journal Volume: 9:3
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CADMIUM; BIOLOGICAL EFFECTS; METALLOTHIONEIN; BIOSYNTHESIS; CHEMICAL COMPOSITION; AMINO ACIDS; KIDNEYS; LIQUID COLUMN CHROMATOGRAPHY; LIVER; MOLECULAR WEIGHT; MUCOUS MEMBRANES; ORAL ADMINISTRATION; RATS; SMALL INTESTINE; ANIMALS; BODY; CARBOXYLIC ACIDS; CHROMATOGRAPHY; DIGESTIVE SYSTEM; ELEMENTS; GASTROINTESTINAL TRACT; GLANDS; INTESTINES; MAMMALS; MEMBRANES; METALLOPROTEINS; METALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RODENTS; SEPARATION PROCESSES; SYNTHESIS; VERTEBRATES; 560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)

Citation Formats

Taguchi, T, and Nakamura, K. Isolation and properties of cadmium-binding protein induced in rat small intestine by oral administration of cadmium. United States: N. p., 1982. Web. doi:10.1080/15287398209530173.
Taguchi, T, & Nakamura, K. Isolation and properties of cadmium-binding protein induced in rat small intestine by oral administration of cadmium. United States. doi:10.1080/15287398209530173.
Taguchi, T, and Nakamura, K. Mon . "Isolation and properties of cadmium-binding protein induced in rat small intestine by oral administration of cadmium". United States. doi:10.1080/15287398209530173.
@article{osti_5299835,
title = {Isolation and properties of cadmium-binding protein induced in rat small intestine by oral administration of cadmium},
author = {Taguchi, T and Nakamura, K},
abstractNote = {The chromatographic properties and amino acid composition of Cd-binding protein induced in the rat small intestine by oral administration of Cd were examined. When the intestinal mucosal cytosol from rats treated orally with Cd was chromatographed on Sephadex G-75, Cd in the intestinal mucosa was bound 95.5% to protein of molecular weight about 10,000. A DEAE Sephadex A025 chromatogram of the Cd-binding protein from Sephadex G-75 showed two major and one minor Cd-containing peaks as hepatic and renal metallothionein. The amino acid composition of the two major Cd peaks resembled that of metallothionein from other materials examined. These results indicate that the low-molecular-weight Cd-binding proteins in the small intestinal mucosa meet the criteria for classification as metallothionein, and suggest that induced intestinal Cd-binding protein may be capable of preventing the transport of orally administered Cd from the intestinal mucosa into the other organs.},
doi = {10.1080/15287398209530173},
journal = {J. Toxicol. Environ. Health; (United States)},
number = ,
volume = 9:3,
place = {United States},
year = {1982},
month = {3}
}