Effect of phase ratio and column type on retention in SFC (supercritical fluid chromatography)

Technical Report · Sat Aug 01 04:00:00 EDT 1987

OSTI ID:7171534

Yonker, C R; Smith, R D

Extensive discussion continues regarding the relative merits of capillary and packed columns for supercritical fluid chromatography (SFC). Columns of interest typically include commercially available 50-..mu..m i.d. capillaries having an /approximately/0.25-..mu..m film thickness and packed columns containing 3-..mu..m or 5-..mu..m diameter developed for HPLC. It has been demonstrated that packed columns can offer advantages in terms of speed because the properties of fluids high linear velocities to be utilized, but the lower pressure drop with open tubular capillary columns allows a greater number of effective theoretical plates through the use of longer columns. Much discussion has centered about the comparison of columns under various operating conditions of practical interest (e.g., pressure programming for capillaries or isobaric operation for packed columns). It is the purpose of this report to show the importance of column phase ratio (i.e., the ratio of stationary to mobile phase volumes. V/sub s/ and V/sub m/, respectively in the selection of columns for specific SFC applications.

• Research Organization: Pacific Northwest Lab., Richland, WA (USA)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 7171534
• Report Number(s): PNL-SA-15206; ON: DE88013119
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
ADSORPTION
AROMATICS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
CHROMATOGRAPHY
COLUMN PACKING
CONDENSED AROMATICS
FLOW RATE
HYDROCARBONS
MOLECULAR WEIGHT
NAPHTHALENE
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PACKINGS
PHENANTHRENE
SEPARATION PROCESSES
SOLUTES
SORPTION
SUPERCRITICAL FLUID CHROMATOGRAPHY