Rapid and efficient capillary column supercritical fluid chromatography with mass spectrometric detection

Journal Article · Thu Nov 01 04:00:00 EST 1984 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00277a048· OSTI ID:5786983

Smith, R D; Kalinoski, H T; Udseth, H R; Wright, B W

Capillary column supercritical fluid chromatography (SFC) using small diameter columns (approx. 50 ..mu..m i.d.) has been demonstrated to provide efficient high-resolution separations. Microparticle packed column SFC has previously been demonstrated to allow very rapid moderate resolution separations, but the large pressure drop required by this approach does not allow effective pressure programming for fast separations to be obtained with capillary column SFC using high-pressure programming rates of up to 50 bar/min and provide advantages compared to packed column methods when the components to be separated span a wide molecular weight range. This approach complements the high-resolution capability of capillary SFC obtained using longer columns and provides increased sensitivity and narrow, nearly constant peak widths ( less than or equal to 6 s), which are ideally compatible with mass spectrometry (MS). Detection limits were found to be approx. 0.1 pg for a series of biphenyl compounds using single ion monitoring. The application of capillary SFC/MS for both rapid and high-resolution separations is demonstrated for standard mixtures and a complex coal tar extract.

• Research Organization: Pacific Northwest Lab., Richland, WA
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 5786983
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 56:13; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
010600 -- Coal
Lignite
& Peat-- Properties & Composition
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400104 -- Spectral Procedures-- (-1987)
400105* -- Separation Procedures
AROMATICS
BIPHENYL
BLOOD VESSELS
BODY
CAPILLARIES
CARDIOVASCULAR SYSTEM
CHEMICAL ANALYSIS
CHROMATOGRAPHY
COAL TAR OILS
DATA
EXPERIMENTAL DATA
HYDROCARBONS
INFORMATION
MASS SPECTROSCOPY
NUMERICAL DATA
OILS
ORGANIC COMPOUNDS
ORGANS
OTHER ORGANIC COMPOUNDS
PRESSURE DEPENDENCE
QUANTITATIVE CHEMICAL ANALYSIS
SEPARATION PROCESSES
SPECTROSCOPY
SUPERCRITICAL FLUID CHROMATOGRAPHY
TRACE AMOUNTS