Pressure programmed microbore column supercritical fluid chromatography-mass spectrometry for the determination of organophosphorus insecticides

Journal Article · Tue Mar 15 04:00:00 EST 1988 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00157a007· OSTI ID:7005541

Kalinoski, H T; Smith, R D

The use of the high flow rate (HFR) interface for supercritical fluid chromatography-mass spectrometry (SFC-MS) is shown to allow operation under conditions which provide efficient pressure programmed separations with appropriate microbore (packed) HPLC columns. The combined advantages of selectivity offered by the microparticle-packed stationary phase and variable solvating power of the supercritical fluid are fully utilized in this approach. The greater sample loadings and lower detection limits possible using packed columns (based on concentration of sample in the injection solvent) compared with commercially available capillary columns are demonstrated for the determination of a series of organophosphorus insecticides. Low concentrations of polar fluid modifiers, generally required for high-quality separations in packed-column SFC, also function as mild chemical ionization reagents. Broad classes of thermally labile, higher molecular weight, moderately polar pesticides are amenable to identification by SFC-MS, which provides a sensitive, selective, and broadly applicable technique for the identification of pesticide compounds with detection limits in the part-per-billion range.

• Research Organization: Pacific Northwest Lab., Richland, WA (USA)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 7005541
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 60:6; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102 -- Chemical & Spectral Procedures
400105* -- Separation Procedures
BLOOD VESSELS
BODY
CAPILLARIES
CARDIOVASCULAR SYSTEM
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DATA
EFFICIENCY
EXPERIMENTAL DATA
FLOW RATE
INFORMATION
INSECTICIDES
INTERFACES
MASS SPECTROSCOPY
MATERIALS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PESTICIDES
PRESSURE DEPENDENCE
QUANTITATIVE CHEMICAL ANALYSIS
SEPARATION PROCESSES
SPECTROSCOPY
SUPERCRITICAL FLUID CHROMATOGRAPHY
TOXIC MATERIALS
TRACE AMOUNTS