Mass spectrometer interface for microbore and high flow rate capillary supercritical fluid chromatography with splitless injection
Improved methods for interfacing microbore and capillary supercritical fluid chromatography (SFC) with mass spectrometry, using a high flow rate interface with splitless injection, are described. The new interface incorporated a mechanically pumped expansion region behind the chemical ionization repeller electrode, allowing higher gas flow rates (100-200 mg of CO/sub 2//min) than previous interfaces and providing an improvement over direct pumping of the ion source in terms of background contributions, ionization efficiency, and sensitivity. The effects of sample flow rate, restrictor geometry, and effluent heating were studied relevant to the transfer and detection of nonvolatile analytes. Separations are compared both microbore and capillary columns for a polarity test mixture, pesticides, and the nonionic surfactant Triton X-100 polymer using supercritical carbon dioxide and carbon dioxide modified with methanol. Microbore columns were shown to have greater limitations for pressure programming, and superior results were obtained with capillary columns.
- Research Organization:
- Pacific Northwest Lab., Richland, WA
- DOE Contract Number:
- AC06-76RL01830
- OSTI ID:
- 7006351
- Journal Information:
- Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 59:1; ISSN ANCHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
400105* -- Separation Procedures
ALCOHOLS
BLOOD VESSELS
BODY
CAPILLARIES
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARDIOVASCULAR SYSTEM
CHALCOGENIDES
CHROMATOGRAPHY
DATA
EXPERIMENTAL DATA
FLOW RATE
HERBICIDES
HYDROXY COMPOUNDS
INFORMATION
INJECTION
INTAKE
MASS SPECTROSCOPY
METHANOL
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PESTICIDES
POLAR COMPOUNDS
SEPARATION PROCESSES
SPECTROSCOPY
SUPERCRITICAL FLUID CHROMATOGRAPHY
SURFACTANTS