Applications of SFC-MS to toxins and compounds of biological interest
The principal applications of supercritical fluid chromatography (SFC) are for compounds that are thermally labile, of low volatility, and of low to moderate polarity. Many compounds of biological and pharmaceutical interest are defined by these criteria. We have examined selected compounds of moderate to high molecular weight with biological activity. We have used the HFR interface with CO/sub 2/ as the mobile phase with from 2 to 5% modifier. One class of compounds potentially addressable by SFC-MS are cyclic peptides. These polar compounds require the use of polar fluid modifiers with CO/sub 2/ as the mobile phase. The ionic nature of the more hydrophilic portions of such polypeptides can sometimes be shielded, allowing solvation in supercritical fluid solvents. Cyclosporin A was successfully separated by SFC-MS using a microbore column using a 2% methanol modifier. The compound was also successfully separated with a capillary column. With the capillary column 2% 2-propanol was adequate, whereas with the microbore column more polar methanol was needed. The mass spectra were identical in both cases. We also analyzed the sodium salt of two ionic polyethers, digitoxigenin, digoxigenin, and cymarin. Results are briefly discussed. 5 refs.
- Research Organization:
- Pacific Northwest Lab., Richland, WA (USA)
- DOE Contract Number:
- AC06-76RL01830
- OSTI ID:
- 7007067
- Report Number(s):
- PNL-SA-15741; CONF-8806199-3; ON: DE88014768
- Country of Publication:
- United States
- Language:
- English
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400102* -- Chemical & Spectral Procedures
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIGENS
BIOLOGICAL MATERIALS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
CHROMATOGRAPHY
MASS SPECTRA
MASS SPECTROMETERS
MATERIALS
MEASURING INSTRUMENTS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
SEPARATION PROCESSES
SPECTRA
SPECTROMETERS
SUPERCRITICAL FLUID CHROMATOGRAPHY
TOXIC MATERIALS
TOXINS