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(/sup 3/H)-ouabain binding sites and (Na/sup +/ + K/sup +/)ATPase activity in heart of rats fed cholesterol

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:7135109
; ;
The purpose of this investigation was to determine the effects of cholesterol on the characteristics of ouabain binding sites and (Na/sup +/ + K/sup +/)ATPase activity in heart. Three groups of male, weanling, Sprague-Dawley rats were fed for 5 weeks diets containing 0, 1 or 2% cholesterol. Membranes were prepared from deoxycholate-treated heart homogenates by differential centrifugation and assayed for ouabain binding and (Na/sup +/ + K/sup +/)ATPase activity. Membranes were incubated with (/sup 3/H)-ouabain in the presence of 10 mM Tris-HCl buffer (pH 7.4) and rapidly filtered on glass fiber filters, GF/A. Non-specific binding was measured in the presence of 6 mM non-labeled ouabain. Concentration of (/sup 3/H)-ouabain binding sites (B/sub max/) was decreased and the binding affinity was increased in the membranes of rats fed 2% cholesterol. The ouabain-sensitive (Na/sup +/ + K/sup +/)ATPase activity was 50-75% lower in membranes prepared from heart of rats fed cholesterol. The Mg/sup 2 +/-ATPase activity was not changed by dietary cholesterol. The results suggest that cholesterol feeding decreases the number of (Na/sup +/ + K/sup +/)ATPase units and allosterically modifies the enzyme.
Research Organization:
Louisiana State Univ. Medical Center, New Orleans
OSTI ID:
7135109
Report Number(s):
CONF-8604222-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
AFFINITY
ALKALI METAL COMPOUNDS
ANIMALS
ATP-ASE
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CELL MEMBRANES
CHOLESTEROL
DRUGS
ENZYME ACTIVITY
ENZYMES
GLYCOSIDES
HEART
HYDROLASES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
ORGANIC COMPOUNDS
ORGANS
OUABAIN
PHOSPHOHYDROLASES
POTASSIUM COMPOUNDS
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SODIUM COMPOUNDS
STEROIDS
STEROLS
STROPHANTHINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES