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Decrease in Na+,K+-ATPase activity and (/sup 3/H)ouabain binding sites in sarcolemma prepared from hearts of spontaneously hypertensive rats

Journal Article · · Hypertension (Dallas); (United States)
OSTI ID:6662046
; ; ; ; ; ;
Na+,K+-ATPase activity, phosphorylation, and (/sup 3/H)ouabain binding in sarcolemma isolated from spontaneously hypertensive rat (SHR) hearts were compared to the same parameters in sarcolemma from normotensive rat (WKY) hearts. Sarcolemma prepared from SHR heart contained significantly less ouabain-inhibitable ATPase activity than sarcolemma from WKY heart. No significant differences in sarcolemmal protein content or recovery were noted between the two groups. The numbers of phosphorylation sites and ouabain binding sites were lower for SHR hearts than for WKY hearts. The KD values for ouabain binding were the same (0.30 muM) in cardiac sarcolemma of SHR and WKY. The I50 values for inhibition by ouabain of Na+,K+-ATPase were also the same for both groups (SHR . 49 microM; WKY . 44 microM). These data suggest that the decrease of cardiac sarcolemmal Na+,K+-ATPase activity in SHR hearts is due to a decrease in the number of active sites.
OSTI ID:
6662046
Journal Information:
Hypertension (Dallas); (United States), Journal Name: Hypertension (Dallas); (United States) Vol. 5:5; ISSN HPRTD
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ALKALI METAL COMPOUNDS
ATP-ASE
BODY
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CATIONS
CHARGED PARTICLES
CHEMICAL REACTIONS
DISEASES
DRUGS
ENZYME ACTIVITY
ENZYMES
GLYCOSIDES
HEART
HYDROLASES
HYPERTENSION
IN VITRO
IONS
LABELLED COMPOUNDS
MEMBRANES
MUSCLES
ORGANIC COMPOUNDS
ORGANS
OUABAIN
PHOSPHOHYDROLASES
PHOSPHORYLATION
POTASSIUM COMPOUNDS
RECEPTORS
SODIUM COMPOUNDS
STROPHANTHINS
SYMPTOMS
TRITIUM COMPOUNDS
VASCULAR DISEASES