Stimulation of ( sup 3 H)ouabain binding to rat synaptosomal (Na sup + + K sup + )-ATPase by aluminum

Conference · Mon Mar 11 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5263746

Caspers, M L; Dow, M J; Kwaiser, T M ^[1]

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1. Univ. of Detroit, MI (United States)

The objective of this study was to investigate the effect of aluminum on the (Na{sup +} + K{sup +})-ATPase. Synaptosomes were prepared from the cerebral cortices of adult, male Sprague-Dawley rats. The stimulation of ({sup 3}H)ouabain binding to the high affinity isoform of the (Na{sup +} + K{sup +})-ATPase produced by AlCl{sub 3} developed slowly, with a maximum effect observed after a 40 min preincubation. AlCl{sub 3} produced a 26.5% stimulation in ({sup 3}H)ouabain binding to the synaptosomal (Na{sup +} + K{sup +})-ATPase and this stimulation increased to 33.3% at 100 {mu}M. Scatchard analysis of ({sup 3}H)ouabain binding data in the presence of 100 {mu}M AlCl{sub 3} yielded a B{sub max} of 79.4 {plus minus} 3.5 pmol/mg protein, significantly elevated from the B{sub max} value obtained in the absence of aluminum. The K{sub D}values were similar in the presence or absence of aluminum. In summary, aluminum affects the functioning of the synaptosomal (Na{sup +} + K{sup +})-ATPase. This may contribute, at least in part, to the disruption of neuronal function associated with disorders where elevated aluminum content in the CNS is noted.

• OSTI ID: 5263746
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:4
• Country of Publication: United States
• Language: English

Related Subjects

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560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID ANHYDRASES
ALUMINIUM
ANIMAL CELLS
ANIMALS
ATP-ASE
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
DRUGS
ELEMENTS
ENZYME ACTIVITY
ENZYMES
GLYCOSIDES
HYDROGEN COMPOUNDS
HYDROLASES
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
METALS
NERVE CELLS
ORGANIC COMPOUNDS
OUABAIN
PHOSPHOHYDROLASES
PROTEINS
RATS
REACTION KINETICS
RODENTS
SOMATIC CELLS
STROPHANTHINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES