Cytochemical and radioautographic identification of cells induced to synthesize hemoglobin

Journal Article · Sat Jun 01 04:00:00 EDT 1974 · Blood; (United States)

OSTI ID:7126158

Rosse, C; Trotter, J A

In order to facilitate the identification of bone marrow cells in which hemoglobin synthesis is initiated, erythropoiesis was first suppressed in guinea pigs through the induction of posthypoxic polycythemia, and then it was restimulated by bleeding and reexposure to hypoxia. Hemoglobin synthesis was detected with /sup 55/Fe incorporation on radioautographs, and its presence was demonstrated in the light microscope with the benzidine reaction and absorption of monochromatic light at lambda 4046 A. In the electron microscope, hemoglobin was detected in the cytoplasm by a general increase in electron density after treating the tissue with diaminobenzidine (DAB) and OsO/sub 4/. Densitometric measurements were carried out on electronmicroscopic negatives, using reticular cell cytoplasm as a base line. In normal marrow, proerythroblasts were the earliest cells in which hemoglobin could be detected, but during the early phase of erythropoietic stimulation, hemoglobin was demonstrated in transitional cells with all the methods employed. Without the specific demonstration of hemoglobin, these cells could not be recognized morphologically as erythroblasts nor could they be distinguished from the precursors of bone marrow small lymphocytes. Transitional cells were numerous in the marrow at the time of stimulation, and 40 hr later a small number of them were labeled with /sup 55/Fe and synthesized hemoglobin in detectable amounts.

• Research Organization: Univ. of Washington, Seattle
• OSTI ID: 7126158
• Journal Information: Blood; (United States), Journal Name: Blood; (United States) Vol. 43:6; ISSN BLOOA
• Country of Publication: United States
• Language: English

Related Subjects

551000* -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANOXIA
AUTORADIOGRAPHY
BIOSYNTHESIS
BLOOD FORMATION
BONE MARROW CELLS
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
ELECTRON MICROSCOPY
ERYTHROPOIESIS
GLOBIN
GUINEA PIGS
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MAMMALS
MICROSCOPY
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RODENTS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES