Cytochemical and radioautographic analysis of erythropoiesis at the ultrastructural level. [Guinea Pig bone marrow]

Journal Article · Sun Sep 01 04:00:00 EDT 1974 · Am. J. Anat.; (United States)

DOI:https://doi.org/10.1002/aja.1001410104· OSTI ID:7231995

Rosse, C; Trotter, J A

The morphogenetic events of erythroid differentiation in guinea pig bone marrow were studied by electron microscopy. The diaminobenzidine (DAB) reaction and radioautographic detection of Fe/sup 55/ incorporation permitted a more objective identification of erythroid cells than was possible in previous studies. With the combined use of these two methods morphological events of cell maturation could be correlated reliably with cytoplasmic hemoglobin content, thus providing a basis for the description of various maturation stages. The oxidation of DAB by hemoglobin coupled with OsO/sub 4/ treatment results in a generalized increase in cytoplasmic electron opacity proportional to the amount of hemoglobin present. Measurements of cytoplasmic density were carried out on electron microscopic negatives of DAB-reacted specimens that were not stained otherwise. Only those cells were regarded as erythroblasts in which the cytoplasm was at least twice as dense as in recognizable, differentiated, non-erythroid cells, using reticular cell cytoplasm in each specimen as the base line for the comparisons. In bone marrow cells, iron incorporation occurred overwhelmingly for hemoglobin synthesis.The morphological characteristics of each of the maturation stages are described with particular emphasis on the earliest erythroid cells, since the methods employed permit their identification with greater degrees of discrimination and objectivity than is possible with the light microscope or with conventional methods of electron microscopy.

• Research Organization: Univ. of Washington, Seattle
• OSTI ID: 7231995
• Journal Information: Am. J. Anat.; (United States), Journal Name: Am. J. Anat.; (United States) Vol. 141:1; ISSN AJANA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD FORMATION
BODY
BODY FLUIDS
BONE MARROW
BONE MARROW CELLS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL DIFFERENTIATION
CHEMICAL REACTIONS
CHEMISTRY
CONNECTIVE TISSUE CELLS
CYTOPLASM
ELECTRON CAPTURE RADIOISOTOPES
ELECTRON MICROSCOPY
ERYTHROCYTES
ERYTHROPOIESIS
EVEN-ODD NUCLEI
GLOBIN
GUINEA PIGS
HEMATOPOIETIC SYSTEM
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
IRON 55
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MICROSCOPY
MORPHOLOGICAL CHANGES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDATION
PIGMENTS
PORPHYRINS
PROTEINS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
TISSUES
TRACER TECHNIQUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES