Progenitor cells of erythroblasts: an in vitro investigation of erythropoietin-responsive cells of guinea pig bone marrow
The experiments were designed to therst whether erythroblast progenitor cell function could be demonstrated in a morphological cell type designated as transitional cells. Two cell fractions were obtained from the bone marrow of normal and polycythemic guinea pigs. One fraction (F1) was enriched in transitional cells and contained few other cell types which could be considered as candidates for erythropoietin responsive cells (ERC). The other fraction (F2) contained undifferentiated blast cells as well as transitional cells. The effect of human urinary erythropoiesis stimulating factors (ESF) on heme synthesis was compared in these two fractions by measuring /sup 59/Fe incorporation into heme. ESF was more effective in stimulating heme synthesis in guinea pig bone marrow cells than homologous sera obtained from anemic or hypoxic animals. The majority of ERC sedimented in F2, but the stimulation index was comparable in the two fractions. It was confirmed by radioautography that the ESF response in F1 was due to the generation of proerythroblasts and basophilic erythroblasts that incorporated /sup 55/Fe. The generation of these cells in F1 was dependent on the addition of ESF to the cultures, whereas /sup 55/Fe-labeled erythroblasts were recovered from cultures of F2 not supplemented with ESF. ESF induced a proportion of transitional cells to incorporate /sup 55/Fe in both F1 and F2. Transitional cells were the only cell type in which heme synthesis was dependent on ESF. Radioautography with /sup 55/Fe identified a proportion of these cells as ERC in both F1 and F2 fractions of bone marrow obtained from normal and polycythemic guinea pigs. The present studies show that some transitional cells function as progenitors of erythroblasts because they respond to ESF by initiation of heme synthesis and by transformation into the earliest recognizable erythroid cells.
- Research Organization:
- Washington Univ., Seattle
- OSTI ID:
- 5704584
- Journal Information:
- Anat. Rec.; (United States), Journal Name: Anat. Rec.; (United States) Vol. 191:2; ISSN ANREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BIOSYNTHESIS
BLOOD FORMATION
BODY
BODY FLUIDS
BONE MARROW CELLS
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ERYTHROPOIESIS
EVEN-ODD NUCLEI
GUINEA PIGS
HEMATOPOIETIC SYSTEM
HEME
HEMIC DISEASES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IN VITRO
INTERMEDIATE MASS NUCLEI
IRON 55
IRON ISOTOPES
ISOTOPES
MAMMALS
MAN
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
POLYCYTHEMIA
PORPHYRINS
PRIMATES
RADIOISOTOPES
RODENTS
SOMATIC CELLS
STEM CELLS
STIMULATION
SYNTHESIS
TISSUE CULTURES
URINE
VERTEBRATES
WASTES
YEARS LIVING RADIOISOTOPES