BIOLOGICAL ACTIVITY OF BONE MARROW CELLS CULTIVATED IN VITRO IN THE PRESENCE OF RADIOISOTOPES (Fe$sup 59$) (in Italian)
Guinea pig bone marrow was incubated in vitro with increasing amounts of radioactive iron. Fe/sup 59/ uptake was evaluated in erythroblasts and in the cells as a whole; at the same time the functional behavior (proliferation, growing, and production of mature cells) of erythroblasts and granuloblasts of normal bone marrow, bone marrow with erythroblastic hyperplasia after venesection or phenylhydrazine, and irradiated bone marrow was investigated. Radio-iron uptake is proportional to the amount of tracer and it varies with the type of bone marrow investigated. Biological activities of hemopoietic cells appear affected even by very small radiation dosages. Granuloblastic stem is the most sensitivei 0.1 mu c/ml induce a maturative arrest in 70% of the cultures. The data obtained show that in vitro utilization of Fe/sup 59/ is a complicated process. Possible interpretations are discussed. (auth)
- Research Organization:
- Universita, Genoa
- NSA Number:
- NSA-13-019792
- OSTI ID:
- 4229284
- Journal Information:
- Minerva nucleare, Journal Name: Minerva nucleare Vol. Vol: 3
- Country of Publication:
- Country unknown/Code not available
- Language:
- Italian
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