ERYTHROCYTE Fe$sup 59$ UPTAKE AS A FUNCTION OF BONE MARROW DOSE INJECTED IN LETHALLY IRRADIATED MICE
The relation between bone marrow cell dose and 24-hour erythrocyte Fe/ sup 59/ uptake was established in lethally irradiated mice. Erythrocyte Fe/sup 59/ uptake is a function of the dose of bone marrow cells and of the time after irradiation at which Fe/sup 59/ is injected. By choosing appropriate bone marrow doses and times of Fe/sup 59/ injection, the range of cell doses between 5 x 10/ sup 5/ and 2 x 10/sup 7/ was explored. The relation between cell dose and Fe/sup 59/ uptake is linear for Fe/sup 59/ uptakes between 0 to 30%. The steepest line relating Fe/sup 59/ uptake to cell dose is that obtained when Fe/sup 59/ was injected at day 9 and covers the range of 5 x 10/sup 4/to 5 x 10/sup 5/ cells. The curve obtained when iron is injected on day 5 is much flatter and covers the range of 1 x 10/sup 6/ to 2 x 10/sup 7/ cells. Erythropoiesis stimulating factor (ESF) in doses that stimulate erythrocyte Fe/sup 59/ uptake in normal mice has no effect in irradiated, bone marrow-treated nmice. Homologous marrow is slightly less effective, and rat bone marrow markedly (~100 times) less effective in promoting recovery of erythropoieis. The erythrocyte Fe/sup 59/ uptake of mice preimmunized with homologous or rat marrow before irradiation is much lower than that of nonpreimmunized animals. (auth)
- Research Organization:
- Oak Ridge National Lab., Tenn.
- NSA Number:
- NSA-16-012888
- OSTI ID:
- 4797243
- Journal Information:
- Blood (U.S.), Journal Name: Blood (U.S.) Vol. Vol: 19; ISSN BLOOA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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