STUDIES ON THE ONTOGENY OF TRANSPLANTATION ANTIGENS IN MICE

Journal Article · Tue Mar 31 04:00:00 EST 1964 · Annals of the New York Academy of Sciences (U.S.)

DOI:https://doi.org/10.1111/j.1749-6632.1964.tb53596.x· OSTI ID:4019297

Doria, G

The development of transplantation antigens in mice was investigated by studying the capability of hemopoietic tissues to induce homotransplantation immunity as a function of their age. The method used was based on the fact that lethally irradiated mice are able to reject homologous bone marrow if they have been preimmunized to hemopoietic tissues of the same donor strain. Bone-marrow rejection was estimated by measuring /sup 59/Fe uptake in erythocytes of the irradiated recipients. The hemopoietic tissues studied were: C3H fetal (131/2-, 151/2-, 171/2-day-old) liver; neonatal (191/2-day-old) liver; and adult (90-day- old) spleen. The method consisted of injecting different cell numbers of either tissue into groups of LAF/sub 1/ mice. These and noninjected LAF/sub 1/ mice (control group) were given an x-ray total-body dose of 800 r 24 hr later. All the mice were injected with a challenge dose of C3H bone marrow within 2 hr after irradiation, and with a tracer dose of /sup 59/Fe nine days after the bone-marrow injection. Radioactivity was measured in a blood sample taken 24 hr after injection of the isotope. The degree of antigenicity of the tissues studied was estimated by the ratio of the /sup 59/Fe uptake in the group of mice injected with any dose of the tested cells to the /sup 59/Fe uptake in the control group of noninjected mice: the smaller the ratio, the greater the antigenicity. The antigenicity of fetal and neonatal livers, when compared to that of adult spleen as to 100%, were: 0%, 131/2 day; 5%, 151/2 day; 8%, 171/2 day; 16%, 191/2 day. By use of proper controls it was possible to reject the hypothesis that the measured antigenicity was a result of further development of the injected cells, which could have grown in the recipient during the time of the experiment. (auth)

• Research Organization: Oak Ridge National Lab., Tenn.
• Sponsoring Organization: USDOE
• NSA Number: NSA-18-019589
• OSTI ID: 4019297
• Journal Information: Annals of the New York Academy of Sciences (U.S.), Journal Name: Annals of the New York Academy of Sciences (U.S.) Vol. Vol: 114; ISSN ANYAA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AGE
ANTIGENS
BIOLOGY AND MEDICINE
BLOOD
BLOOD CELLS
BONE MARROW
ERYTHROCYTES
FETUSES
IMMUNITY
IRON 59
IRRADIATION
LABELLED COMPOUNDS
LETHAL DOSE
LIVER
MEASURED VALUES
METABOLISM
MICE
NUMERICALS
PERFORMANCE
RADIATION DOSES
RADIOACTIVITY
SAMPLING
SPLEEN
STANDARDS
STATISTICS
TESTING
TISSUES
TRACER TECHNIQUES
TRANSPLANTS
X RADIATION