Effect of syngeneic marrow injection upon recovery in sub- and near-lethally irradiated mice

Journal Article · Thu Jun 01 04:00:00 EDT 1989 · Exp. Hematol.; (United States)

OSTI ID:6068067

Boggs, S S; Boggs, D R; Patrene, K D

Mice were given sub-lethal (200-600 cGy) or near-lethal (800 cGy) whole body irradiation and the effect of injecting syngeneic marrow on subsequent hematopoietic recovery was studied. Marrow cell injection enhanced erythropoietic recovery after sub-lethal irradiation as reflected in hematocrit values and rate of appearance of /sup 59/Fe-labeled red cells in blood. However, this enhanced erythropoiesis was only seen in the spleen, and /sup 59/Fe uptake in marrow was reduced. When the irradiation dose was kept constant and the marrow dose increased from 10(5) to 10(6) to 10(7) cells, there was a somewhat erratic increase in spleen /sup 59/Fe and a decrease in marrow /sup 59/Fe uptake. When marrow cell number was kept constant and the dose of irradiation was increased from 200 to 400 to 600 to 800 cGy, there was an exponential increase in spleen /sup 59/Fe uptake but the marrow /sup 59/Fe uptake changed from depressed after lower doses to increased after 800 cGy. Cell injection after sub-lethal irradiation did not increase or decrease granulocytopoiesis. Injection of irradiated marrow cells also reduced marrow erythropoiesis and this was evident after both sub- and near-lethal irradiation. However, injection of irradiated cells did not increase splenic erythropoiesis. Following splenectomy, the depressed marrow erythropoiesis attending injection of viable cells was virtually eliminated but no increase was seen. These data suggest that the injection of autologous or syngeneic marrow may not be effective as a means of accelerating hematopoietic recovery after irradiation unless near-lethal or lethal dose have been received.

• Research Organization: Univ. of Pittsburgh School of Medicine, PA (USA)
• OSTI ID: 6068067
• Journal Information: Exp. Hematol.; (United States), Journal Name: Exp. Hematol.; (United States) Vol. 17:5; ISSN EXHMA
• Country of Publication: United States
• Language: English

Related Subjects

551001 -- Physiological Systems-- Tracer Techniques
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BLOOD
BLOOD CELLS
BLOOD COUNT
BLOOD FORMATION
BODY
BODY FLUIDS
BONE MARROW CELLS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DOSES
ERYTHROCYTES
ERYTHROPOIESIS
EVEN-ODD NUCLEI
EXTERNAL IRRADIATION
HEMATOPOIETIC SYSTEM
INTERMEDIATE MASS NUCLEI
IRON 59
IRON ISOTOPES
IRRADIATION
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
MEDICINE
MICE
NUCLEI
ORGANS
RADIATION DOSES
RADIOISOTOPES
RADIOSENSITIVITY EFFECTS
RECOVERY
RODENTS
SOMATIC CELLS
SPLEEN
SPLENECTOMY
SUBLETHAL IRRADIATION
SURGERY
TRACER TECHNIQUES
TRANSPLANTS
VERTEBRATES
WEIGHT
WHOLE-BODY IRRADIATION