Effect of caffeine on the ultraviolet light induction of SV40 virus from transformed hamster cells

Journal Article · Thu Jan 01 00:00:00 EST 1976 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(76)90408-6· OSTI ID:7120555

Zamansky, G B; Kleinman, L F; Little, J B; Black, P H; Kaplan, J C

The effect of caffeine on the uv light induction of SV40 virus from two transformed hamster cell lines heterogeneous for the induction of infectious virus was studied. The amount of virus induced was significantly increased in both cell lines when exposure to uv light was followed by treatment with caffeine. Caffeine in the absence of uv irradiation did not stimulate virus induction, nor did it stimulate SV40 replication in a lytic infection. There was an apparent difference in the concentrations of caffeine which maximally stimulated SV40 virus induction in the two cell lines. This effect could not be explained by differences in cell survival after exposure to uv light and caffeine. Since caffeine is known to cause the accumulation of gaps formed in DNA during postreplication repair of uv-irradiated rodent cells, our results support the hypothesis that the formation of gaps or breaks in DNA is an important early step in virus induction.

• Research Organization: Massachusetts General Hospital, Boston
• OSTI ID: 7120555
• Journal Information: Virology; (United States), Vol. 73:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CAFFEINE
RADIOSENSITIVITY EFFECTS
VIRUSES
RADIOINDUCTION
ANIMAL CELLS
BIOLOGICAL REPAIR
DNA
IRRADIATION
ULTRAVIOLET RADIATION
BIOLOGICAL RECOVERY
DRUGS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PARASITES
PURINES
RADIATIONS
RECOVERY
REPAIR
XANTHINES
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)