SPECT (single photon emission computed tomography) assay of radiolabeled monoclonal antibodies

Technical Report · Sun Apr 01 04:00:00 EDT 1990

DOI:https://doi.org/10.2172/7100325· OSTI ID:7100325

Jaszczak, R J

The accurate determination of the biodistribution of radiolabeled monoclonal antibodies (MoAbs) is important for calculation of dosimetry and evaluation of pharmacokinetic variables such as antibody dose and route of administration. A major long-term objective of this proposal is to determine the utility of single photon emission computed tomography (SPECT) for quantifying the biodistribution of monoclonal antibodies labeled with the clinically relevant radionuclides iodine-123 (I-123) and indium-111 (In-111). The specific aims of this project include the comparison of the effects of three methods of radiolabeling anticarcinoembryonic antigen (CEA-110) --- iodination by Iodogen, the tin-containing ester methods and an indium chelate method --- on the immunoreactivity and affinity of the antibody. The biodistribution of anti-CEA labeled by the three methods will be compared in tumor bearing and normal mice. Subsequent studies of the pharmacokinetics of I-123 and In-111 labeled anti-CEA will be determined by SPECT in non-human primates. The errors associated with the SPECT measurements will be assessed with Monte Carlo simulations and by scanning phantoms containing I-123 or In-111 activity in regions of uniform and nonuniform attenuation. The ability of SPECT to quantify I-123 and In-111 distributions will be assessed, and new acquisition geometries and reconstruction algorithms for improved quantification will be evaluated. 8 figs., 4 tabs.

• Research Organization: Duke Univ. Medical Center, Durham, NC (USA). Dept. of Radiology
• Sponsoring Organization: DOE/ER
• DOE Contract Number: FG05-89ER60894
• OSTI ID: 7100325
• Report Number(s): DOE/ER/60894-1; ON: DE90012438
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
560162 -- Radionuclide Effects
Kinetics
& Toxicology-- Animals
Plants
Microorganisms
& Cells
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALGORITHMS
ANIMALS
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
BIOASSAY
BIOLOGICAL EFFECTS
COMPARATIVE EVALUATIONS
COMPUTERIZED TOMOGRAPHY
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DIAGNOSTIC USES
DISEASES
DOCUMENT TYPES
DOSIMETRY
ELECTRON CAPTURE RADIOISOTOPES
EMISSION COMPUTED TOMOGRAPHY
HOURS LIVING RADIOISOTOPES
IMAGE PROCESSING
IMMUNOASSAY
IMMUNOLOGY
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 123
IODINE ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
MAMMALS
MATHEMATICAL LOGIC
MINUTES LIVING RADIOISOTOPES
MOCKUP
MONOCLONAL ANTIBODIES
MONTE CARLO METHOD
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
PHANTOMS
PRIMATES
PROCESSING
PROGRESS REPORT
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
RADIONUCLIDE KINETICS
STRUCTURAL MODELS
TOMOGRAPHY
TRACER TECHNIQUES
USES
VERTEBRATES