SPECT assay of radiolabeled monoclonal antibodies

Technical Report · Wed May 01 04:00:00 EDT 1991

OSTI ID:5870347

Jaszczak, R J

The accurate determination of the biodistribution of radiolabeled monoclonal antibodies (MoAbs) is important for calculation of dosimetry and evaluation of pharmacokinetic variables such as antibody dose and route of administration. A major long-term objective of this proposal is to determine the utility of single photon emission computed tomography (SPECT) for quantifying the biodistribution of monoclonal antibodies labeled with the clinically relevant radionuclide iodine-123 (I-123). The pharmacokinetics of I-123 labeled MoAbs will be determined by the SPECT in non-human primates. The errors associated with the SPECT measurements will be assessed with Monte Carlo simulations and by scanning phantoms containing I-123 activity in regions of uniform and nonuniform attenuation. The ability of SPECT to quantify I-123 distributions will be assessed, and new acquisition geometries and reconstruction algorithms for improved quantification will be evaluated. 33 refs.

• Research Organization: Duke Univ. Medical Center, Durham, NC (USA). Dept. of Radiology
• Sponsoring Organization: DOE; USDOE, Washington, DC (USA)
• DOE Contract Number: FG05-89ER60894
• OSTI ID: 5870347
• Report Number(s): DOE/ER/60894-2; ON: DE91012158
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
560162 -- Radionuclide Effects
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& Toxicology-- Animals
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62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALGORITHMS
ANIMALS
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
COMPUTERIZED TOMOGRAPHY
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DIAGNOSTIC USES
DOCUMENT TYPES
DOSIMETRY
ELECTRON CAPTURE RADIOISOTOPES
EMISSION COMPUTED TOMOGRAPHY
HOURS LIVING RADIOISOTOPES
IMAGE PROCESSING
IMMUNOASSAY
IMMUNOLOGY
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 123
IODINE ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
MAMMALS
MATHEMATICAL LOGIC
MINUTES LIVING RADIOISOTOPES
MOCKUP
MONOCLONAL ANTIBODIES
MONTE CARLO METHOD
NUCLEI
ODD-EVEN NUCLEI
PHANTOMS
PRIMATES
PROCESSING
PROGRESS REPORT
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
RADIONUCLIDE KINETICS
STRUCTURAL MODELS
TECHNETIUM 99
TECHNETIUM ISOTOPES
TOMOGRAPHY
TRACER TECHNIQUES
USES
VERTEBRATES
YEARS LIVING RADIOISOTOPES