Elimination of hypoxic protection by 5-thio-D-glucose in multicell spheroids

Journal Article · Fri Dec 01 04:00:00 EST 1978 · Cancer Res.; (United States)

OSTI ID:7097388

Song, C W; Clement, J J; Levitt, S H

The effect of 5-thio-D-glucose on oxic and hypoxic V79-171 Chinese hamster cells was studied in vitro with single cells and multicell spheroids. At concentrations that were not toxic to oxic cells, this compound killed hypoxic cells with a D0 of 1 h with a 5 mM concentration and more rapidly at 10 mM beginning 2 h after incubation at 37 degrees. 5-thio-D-glucose also sensitized hypoxic cells to radiation and protected oxic cells from radiation damage. Multicell spheroids irradiated after incubation with the compound demonstrated increased radiosensitivity. The dramatic increase in multicell spheroid radiosensitivity that resulted from treatment with 5-thio-D-glucose suggests that this compound may be used to increase the effectiveness of radiotherapy by eliminating hypoxic protection.

• Research Organization: Univ. of Minnesota, Minneapolis
• OSTI ID: 7097388
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 38:12; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ANIMAL CELLS
ANOXIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBOHYDRATES
DOSE-RESPONSE RELATIONSHIPS
GLUCOSE
HEXOSES
IN VITRO
IONIZING RADIATIONS
MONOSACCHARIDES
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
SACCHARIDES
SPHEROIDS