Mechanisms for radiation damage in DNA. Progress report, August 1, 1976--October 31, 1977
In this project we have proposed mechanisms for radiation damage to DNA constituents and DNA, and have detailed a series of experiments utilizing electron spin resonance spectrometry to test the proposed mechanisms. In this past year investigations were completed on reactions of the ..pi..-cations of thymidine TMP and 1-methylthymine, charge transfer in aromatic peptide ..pi..-cation radicals, and electron reactions with amino acid anhydrides. A study in which we have made significant progress in this past year is ..pi..-cation radicals in DNA and dinucleoside phosphates. In the first study the cations of thymine derivatives were found to decay by two paths, hydroxyl ion addition and methyl group deprotonation. In the second it was found that ..pi..-cations of aromatic amino acid residues transferred the charge to the carboxyl group in dipeptides. This resulted in decarboxylation. In the last study it was found that the positive charge in DNA localizes mainly on the purine base guanine.
- Research Organization:
- Oakland Univ., Rochester, MI (USA)
- DOE Contract Number:
- EY-76-S-02-2364
- OSTI ID:
- 7094358
- Report Number(s):
- COO-2364-10
- Country of Publication:
- United States
- Language:
- English
Similar Records
Mechanisms for radiation damage in DNA, Progress report, November 1, 1977--October 31, 1978. [Gamma radiation]
Mechanisms for radiation damage in DNA constituents and DNA: reactions of the N/sub 1/-substituted thymine. pi. -cation radicals
Related Subjects
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANHYDRIDES
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DECARBOXYLATION
DNA
ELECTRON SPIN RESONANCE
EPR SPECTROMETERS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INJURIES
KINETICS
MAGNETIC RESONANCE
MEASURING INSTRUMENTS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATION INJURIES
RADICALS
REACTION KINETICS
RESONANCE
RIBOSIDES
SPECTROMETERS
THYMIDINE
THYMINE
URACILS