Mechanisms for radiation damage in DNA. Progress report, August 1, 1975--July 31, 1976

Technical Report · Thu Jan 01 04:00:00 EST 1976

DOI:https://doi.org/10.2172/7190409· OSTI ID:7190409

Sevilla, M D

In this project we have proposed a mechanism for radiation damage to DNA and detailed a series of experiments utilizing electron spin resonance spectrometry to test the proposed mechanisms. In this past year several investigations have been completed or are nearing completion. These investigations are an ESR study of the N/sub 1/-substituted thymine ..pi..-cation radicals; studies of electron reactions with amino acid anhydrides; and ESR and pulse radiolytic studies of electron transfer in dinucleoside phosphate anions. Studies which we have made significant progress on in this past year are positive ion radicals of the dinucleoside phosphates, and reactions of the ..pi..-cations of thymine derivatives. In the first study the spin density distribution in the cation radicals of thymidine and thymidine-5/sup 1/-monophosphate have been elucidated. Couplings are found to the ribose group in these radicals. It is believed that these results will aid the identification of cation radicals in DNA. In study 2, the results indicate that these cyclic anhydrides can undergo reductive deamination. In study 3, the results show the order of electron affinities of the DNA bases to be thymine approximately equal to cytosine greater than adenine approximately equal to guanine.

• Research Organization: Oakland Univ., Rochester, Mich. (USA)
• OSTI ID: 7190409
• Report Number(s): COO-2364-9
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
DNA
ELECTRON SPIN RESONANCE
ELECTRON TRANSFER
ELECTRONS
ELEMENTARY PARTICLES
FERMIONS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INJURIES
LEPTONS
MAGNETIC RESONANCE
MEASURING INSTRUMENTS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PULSE TECHNIQUES
PYRIMIDINES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATION INJURIES
RADICALS
RADIOLYSIS
RESONANCE
RIBOSIDES
SPECTROMETERS
THYMINE
URACILS