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Mechanisms for radiation damage in DNA constituents and DNA: reactions of the N/sub 1/-substituted thymine. pi. -cation radicals

Conference ·
OSTI ID:7218713

Reactions of the N/sub 1/-substituted thymine ..pi..-cation radicals were investigated by ESR in several aqueous glasses. In 8M NaOD (NaOH) spectra suggestive and OD/sup -/(OH/sup -/) addition to position 6 in the ..pi..-cations of 1-methylthymine, and thymidine were found immediately after uv photolysis at 77/sup 0/K. Production of the same radicals by electron attachment to 1-methyl-5-bromo-6-hydroxythymine, and 5-bromo-6-hydroxythymidine in 8M NaOD confirms the OD/sup -/ addition mechanism. Results found for these brominated compounds in 12M LiCl (D/sub 2/O) after electron attachment show that the ..cap alpha../sub 6/ (H) splitting was sensitive to changes in substituents at position 1 as well as changes in environment. This variation in splitting is shown to be accounted for by small conformational changes in the radicals. In 8M NaClO/sub 4/ ..pi..-cations of the substituted thymines gave evidence for both deprotonation and OD- addition.

Research Organization:
Oakland Univ., Rochester, MI (USA). Dept. of Chemistry
DOE Contract Number:
EY-76-S-02-2364
OSTI ID:
7218713
Report Number(s):
COO-2364-11; CONF-770366-1
Country of Publication:
United States
Language:
English