Mechanisms for radiation damage in DNA constituents and DNA: reactions of the N/sub 1/-substituted thymine. pi. -cation radicals

Conference · Sat Jan 01 04:00:00 EST 1977

OSTI ID:7218713

Sevilla, M D; Engelhardt, M L

Reactions of the N/sub 1/-substituted thymine ..pi..-cation radicals were investigated by ESR in several aqueous glasses. In 8M NaOD (NaOH) spectra suggestive and OD/sup -/(OH/sup -/) addition to position 6 in the ..pi..-cations of 1-methylthymine, and thymidine were found immediately after uv photolysis at 77/sup 0/K. Production of the same radicals by electron attachment to 1-methyl-5-bromo-6-hydroxythymine, and 5-bromo-6-hydroxythymidine in 8M NaOD confirms the OD/sup -/ addition mechanism. Results found for these brominated compounds in 12M LiCl (D/sub 2/O) after electron attachment show that the ..cap alpha../sub 6/ (H) splitting was sensitive to changes in substituents at position 1 as well as changes in environment. This variation in splitting is shown to be accounted for by small conformational changes in the radicals. In 8M NaClO/sub 4/ ..pi..-cations of the substituted thymines gave evidence for both deprotonation and OD- addition.

• Research Organization: Oakland Univ., Rochester, MI (USA). Dept. of Chemistry
• DOE Contract Number: EY-76-S-02-2364
• OSTI ID: 7218713
• Report Number(s): COO-2364-11; CONF-770366-1
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400600* -- Radiation Chemistry
ALKALI METAL COMPOUNDS
AQUEOUS SOLUTIONS
AZINES
CATIONS
CHARGED PARTICLES
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
CHLORIDES
CHLORINE COMPOUNDS
DECOMPOSITION
DEUTERIUM COMPOUNDS
DISPERSIONS
DNA
ELECTROMAGNETIC RADIATION
ELECTRON SPIN RESONANCE
GLASS
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXIDES
HYDROXY COMPOUNDS
IONS
LITHIUM CHLORIDES
LITHIUM COMPOUNDS
LITHIUM HALIDES
LOW TEMPERATURE
MAGNETIC RESONANCE
MIXTURES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PERCHLORATES
PHOTOCHEMICAL REACTIONS
PHOTOLYSIS
PYRIMIDINES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
RESONANCE
SODIUM COMPOUNDS
SODIUM HYDROXIDES
SODIUM PERCHLORATES
SOLUTIONS
THYMINE
ULTRAVIOLET RADIATION
URACILS