Chromosomal aberrations induced by in vitro irradiation: comparisons between human sperm and lymphocytes
Journal Article
·
· Environ. Mol. Mutagen.; (United States)
Types and frequencies of structural aberrations in human sperm and lymphocyte chromosomes from one donor were compared after in vitro irradiation with 100, 200, and 400 rad in order to determine if cells with dramatically different chromatin configurations are similarly affected and to investigate the feasibility of using lymphocytes as surrogates for germ cells in risk estimation. Sperm chromosomes were analyzed after fusion with eggs from the golden hamster. Total frequencies of induced aberrations were similar in the two cell types. However, the relative frequencies of rejoined lesions (dicentrics), compared with unrejoined lesions (chromosome breaks and acentric fragments), were different. At the three doses tested, a constant ratio of 5 dicentrics in lymphocytes for every dicentric in sperm was induced. Conversely, for every chromosome break or acentric fragment induced in lymphocytes, 1.7 such events were induced in sperm at the three doses tested.
- Research Organization:
- Lawrence Livermore National Lab., CA (USA)
- OSTI ID:
- 7092130
- Journal Information:
- Environ. Mol. Mutagen.; (United States), Journal Name: Environ. Mol. Mutagen.; (United States) Vol. 12:2; ISSN ENMUE
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMOSOMAL ABERRATIONS
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
DOSES
GAMETES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GERM CELLS
HAMSTERS
IN VITRO
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MUTATION FREQUENCY
MUTATIONS
PRIMATES
RADIATION DOSES
RADIATION EFFECTS
RADIOINDUCTION
RODENTS
SOMATIC CELLS
SPERMATOZOA
VERTEBRATES
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMOSOMAL ABERRATIONS
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
DOSES
GAMETES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GERM CELLS
HAMSTERS
IN VITRO
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MUTATION FREQUENCY
MUTATIONS
PRIMATES
RADIATION DOSES
RADIATION EFFECTS
RADIOINDUCTION
RODENTS
SOMATIC CELLS
SPERMATOZOA
VERTEBRATES