Detection of chromosomal abnormalities in human sperm

Conference · Wed Jun 19 04:00:00 EDT 1985

OSTI ID:5483732

Brandriff, B; Gordon, L; Ashworth, A K; Watchmaker, G; Carrano, A V

A new technology developed by Rudak, et al. for examining the chromosomal constitution of human sperm through fusion with eggs from the Syrian hamster was used to obtain baseline data on the types and frequencies of aberrations in sperm of normal men. The frequency of structural aberrations in 2724 sperm chromosome karyotypes from the 13 healthy non-exposed donors ranged from 2 to 15.8%, demonstrating significant interindividual variability. The most frequently occurring aberrations were chromosome breaks, followed by acentric fragments, chromatid exchanges, chromatid breaks, dicentrics and translocations, chromosome deletions and duplications, inversions, and chromatid deletions. Two donors previously reported had one cell each with multiple chromatid exchanges and breaks. In addition, the oldest donor, AA, had 5 cells out of 124 examined with multiple breaks and rearrangements too extensive to completely identify. 17 refs., 2 tabs.

• Research Organization: Lawrence Livermore National Lab., CA (USA)
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 5483732
• Report Number(s): UCRL-92835; CONF-8506137-3; ON: DE85014178
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANEUPLOIDY
ANIMALS
CHROMOSOMAL ABERRATIONS
DATA
EXPERIMENTAL DATA
FERTILIZATION
GAMETES
GERM CELLS
HAMSTERS
INFORMATION
MAMMALS
MAN
MUTATIONS
NUMERICAL DATA
PLOIDY
PRIMATES
RODENTS
SCREENING
SPERMATOZOA
VERTEBRATES