Interactions with insulin and dexamethasone in net synthesis of albumin and acute-phase proteins

Journal Article · Thu Jan 01 04:00:00 EST 1976 · Diabetes; (United States)

OSTI ID:7086858

Miller, L L

The isolated rat liver perfused for 12 hours at pH 7.10 with a suspension of bovine erythrocytes in Krebs--Ringer bicarbonate buffer containing 3% bovine serum albumin has been used as a test system to study effects of glucagon and of dexamethasone in the presence and absence of insulin on net biosynthesis of rat serum albumin, fibrinogen, ..cap alpha../sub 1/-acid glycoprotein, ..cap alpha../sub 2/-(acute phase) globulin, and haptoglobin. Quantitative measurement of perfusate glucose, amino acid nitrogen, and urea affords a basis for determining net glucose and nitrogen balance in the perfusion system. Although the dose of dexamethasone (total 1.0 ..mu..g) used was insufficient to induce synthesis of ..cap alpha../sub 2/-acute phase globulin, net syntheses of albumin, fibrinogen, ..cap alpha../sub 1/-acid glycoprotein, and haptoglobin were increased. Glucagon given with dexamethasone depressed albumin and haptoglobin synthesis markedly, but not that of fibrinogen and ..cap alpha../sub 1/-acid glycoprotein. Glucagon with dexamethasone markedly enhanced ureogenesis and glycogenolysis and elicited an exaggerated negative nitrogen balance. The unfavorable effects of glucagon on albumin and haptoglobin synthesis and on nitrogen balance were reversed by giving insulin simultaneously. It is emphasized that insulin is essential for positive nitrogen balance.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 7086858
• Journal Information: Diabetes; (United States), Journal Name: Diabetes; (United States) Vol. 25:Suppl. 2; ISSN DIAEA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ALBUMINS
AMIDES
AMINO ACIDS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACIDS
CORTICOSTEROIDS
CRYOGENIC FLUIDS
DEXAMETHASONE
DIGESTIVE SYSTEM
ELEMENTS
ERYTHROCYTES
FLUIDS
GLANDS
GLOBULINS
GLUCAGON
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
INSULIN
KETONES
LIVER
MAMMALS
METABOLISM
NITROGEN
NONMETALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDE HORMONES
PEPTIDES
PERFUSED ORGANS
POLYPEPTIDES
PREGNANES
PROTEINS
RATS
RODENTS
STEROID HORMONES
STEROIDS
SYNTHESIS
UREA
VERTEBRATES