Net biosynthesis of Antithrombin III by the isolated rat liver perfused for 12--24 hours. Compared with rat fibrinogen and. cap alpha. -2 (acute phase) globulin, Antithrombin III is not an acute phase protein

Technical Report · Sun Jan 01 04:00:00 EST 1978

DOI:https://doi.org/10.2172/6230583· OSTI ID:6230583

Owens, M. R.; Miller, L. L.

Antithrombin III- heparin cofactor has been isolated from normal rat plasma, purified to homogeniety on acrylamide gel electrophoresis and used to prepare a monospecific antiserum in rabbits. Measurements of rat AT-III were made by a single radial immunodiffusion assay. Net synthesis of AT-III was investigated during 12 or 24 hour perfusions of the isolated rat liver. In perfusions performed under basal conditions cumulative synthesis of AT-III was observed to occur at a rate sufficient to replace the total circulating plasma AT-III in about 6 hours. In perfusions performed under full supplementation conditions which greatly enhanced synthesis of fibrinogen and ..cap alpha..-2 (acute phase) globulin (known acute phase reactant proteins) net synthesis of AT-III was not significantly greater than that observed in control perfusions. Although these prolonged perfusion studies conclusively demonstrate net synthesis of AT-III by the isolated rat liver, they afford no evidence that this protein is an acute phase reactant.

• Research Organization: Rochester Univ., NY (USA). School of Medicine and Dentistry; Saint Mary's Hospital, Rochester, NY (USA). Dept. of Medicine
• DOE Contract Number: EY-76-C-02-3490
• OSTI ID: 6230583
• Report Number(s): UR-3490-1578
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
ANTICOAGULANTS
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BLOOD COAGULATION FACTORS
BODY
CARBOHYDRATES
DATA
DATA FORMS
DIGESTIVE SYSTEM
DRUGS
EXPERIMENTAL DATA
FIBRINOGEN
GLANDS
GLOBULINS
GRAPHS
HEPARIN
INFORMATION
KINETICS
LIVER
MAMMALS
MUCOPOLYSACCHARIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PERFUSED TISSUES
POLYSACCHARIDES
PROTEINS
RATS
REACTION KINETICS
RODENTS
SACCHARIDES
SYNTHESIS
TISSUES
VERTEBRATES