Ionizing radiation-induced expression of the genes associated with the acute response to injury in the rat

Journal Article · Tue Aug 01 04:00:00 EDT 1995 · Radiation Research

DOI:https://doi.org/10.2307/3579156· OSTI ID:121756

Magic, Z; Matic-Ivanovic, S; Savic, J ^[1]

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1. Institute for Biologica Research, Belgrade (Yugoslavia); and others

Total-body irradiation of rats with doses ranging from an LD{sub 10/30} to an LD{sub 100/30} induced a dose-dependent increase in the concentration of serum protein associated with the acute response to the irradiation. However, this increase was reached at a later time and was not as pronounced as described previously during the typical acute phase of the response found experimentally. The greatest increase in the serum concentrations of acute-phase proteins was found from the third to the seventh days postirradiation. At these times, the serum concentrations of {alpha}{sub 2}-macroglobulin, haptoglobin, fibrinogen and cysteine protease inhibitor were raised from two- to fivefold, whereas {alpha}{sub 1}-acid glycoprotein was increased sixfold. Incorporation of [{sup 35}S]methionine into total serum and acute-phase proteins indicated that the increase in the concentration of the acute-phase proteins was preceded by their de novo synthesis in the liver. The results that were obtained by dot-blot analysis showed that the basic course of change in the relative m RNA concentrations of a{alpha}{sub 1}-acid glycoprotein had an extrahepatic origin. On the basis of these results we concluded that total-body irradiation increased the expression of acute-phase protein genes. 50 refs., 5 figs.

• Sponsoring Organization: USDOE
• OSTI ID: 121756
• Journal Information: Radiation Research, Journal Name: Radiation Research Journal Issue: 2 Vol. 143; ISSN 0033-7587; ISSN RAREAE
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
DOSE-RESPONSE RELATIONSHIPS
FIBRINOGEN
GENE REGULATION
LIVER
METHIONINE
PROTEINS
RADIOINDUCTION
RATS
RESPONSE MODIFYING FACTORS
WHOLE-BODY IRRADIATION