Radioimmunoassay of human homologous prolactin in serum with commercially available reagents. [/sup 125/I tracer technique]

Journal Article · Thu Sep 01 04:00:00 EDT 1977 · Clin. Chem.; (United States)

OSTI ID:7086834

Kao, P C; Jiang, N S; Abboud, C F

A clinically useful and reproducible radioimmunoassay for human homologous prolactin, established with commercially available reagents, was studied and validated. We present detailed conditions for iodination and purification of labeled prolactin and the optimal conditions for the assay. By the method, we found values (..mu..g/liter) as follows for serum prolactin: normal men, 8.9 +- 5.2 (mean +- SD); normal women, 11.8 +- 5.5; normal women taking contraceptive pills, 9.2 +- 5.0; pregnant women in the third trimester, 188 +- 69.5; patients with various diseases other than of the hypothalamic-pituitary axis, 9.3 +- 6.3; in some patients with amenorrhea and galactorrhea of diverse origin, 78.2 +- 87.4; and in some patients with surgically proven pituitary tumor, 1414 +- 1980. Results under provocative testing are also presented for a patient with normal hypothalamic-pituitary function.

• Research Organization: Mayo Clinic and Mayo Foundation, Rochester, MN
• OSTI ID: 7086834
• Journal Information: Clin. Chem.; (United States), Journal Name: Clin. Chem.; (United States) Vol. 23:9; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BLOOD SERUM
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GONADOTROPINS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LTH
NUCLEI
ODD-EVEN NUCLEI
PATIENTS
PEPTIDE HORMONES
PITUITARY HORMONES
PURIFICATION
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
REAGENTS
TRACER TECHNIQUES