Development and characterization of a homologous radioimmunoassay for deer mouse (Peromyscus maniculatus bairdii) prolactin

Journal Article · Mon Dec 05 04:00:00 EST 1983 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(83)90264-3· OSTI ID:5022072

Marr, G A; Colosi, P; Desjardins, C; Talamantes, F

A highly specific and sensitive homologous radioimmunoassay has been developed for the secreted form of prolactin from the deer mouse Peromyscus maniculatus bairdii. Peromyscus serum and pituitary homogenates displayed paralled dilution response curves, and no cross reaction was seen with either mouse prolactin, mouse growth hormone or rat prolactin. The assay was sensitive to 25 picograms per tube and the intra- and inter-assay coefficients of variation were 5 and 3.6%, respectively. In addition, the authors have demonstrated that Peromyscus prolactin does not show parallel displacement in a homologous radioimmunoassay utilized for measuring prolactin in the common laboratory mouse.

• Research Organization: Univ. of California, Santa Cruz
• OSTI ID: 5022072
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 33:23; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALI METAL COMPOUNDS
ANIMALS
BETA DECAY RADIOISOTOPES
BLOOD SERUM
BODY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
GLANDS
GONADOTROPINS
HALIDES
HALOGEN COMPOUNDS
HORMONES
INORGANIC PHOSPHORS
INTERMEDIATE MASS NUCLEI
IODIDES
IODINE 125
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LTH
MAMMALS
MICE
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PEPTIDE HORMONES
PHOSPHORS
PITUITARY GLAND
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RODENTS
SAMPLING
SODIUM COMPOUNDS
SODIUM IODIDES
TRACER TECHNIQUES
VERTEBRATES