The development and characterization of a homologous radioimmunoassay for mouse placental lactogen

Journal Article · Mon Feb 01 04:00:00 EST 1982 · Endocrinology; (United States)

DOI:https://doi.org/10.1210/endo-110-2-668· OSTI ID:5707782

Soares, M J; Colosi, P; Talamantes, F

A highly specific and sensitive homologous radioimmunoassay (RIA) has been developed for mouse placental lactogen (mPL). Mouse pregnancy serum and placental extracts showed parallel dilution-response curves, and no significant cross-reaction was seen with either mouse growth hormone or mouse prolactin. The sensitivity of the assay ranged from 0.5 to 1.0 ng/ml, and the intra- and inter-assay coefficients of variation were 5 and 11%, respectively. By RIA, mPL levels were detectable on day 9 of pregnancy (1 ng/ml) and increased until day 14 (100-150 ng/ml) in both C3H and BALB/c strains. On days 4-18 of pregnancy, mPL levels were maintained (95-125 ng/ml) in C3H mice, while they continued to increase (>250 ng/ml) in BALB/c mice. In both strains, a mid-pregnancy peak in prolactin-like activity was detected by a radioreceptor assay but not by the mPL RIA.

• Research Organization: University of California, Santa Cruz
• OSTI ID: 5707782
• Journal Information: Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 110:2; ISSN ENDOA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FETAL MEMBRANES
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LACTOGENS
MAMMALS
MEMBRANES
MICE
NUCLEI
ODD-EVEN NUCLEI
PLACENTA
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RADIORECEPTOR ASSAY
RODENTS
SENSITIVITY
SPECIFICITY
TRACER TECHNIQUES
VERTEBRATES