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Use of polyethylene glycol in radioimmunoassay of human placental lactogen. [/sup 125/I tracer technique]

Journal Article · · Clin. Chem.; (United States)
OSTI ID:6204404
We describe a rapid radioimmunoassay for human placental lactogen in biological fluids, with use of polyethylene glycol to separate free from antibody-bound placental lactogen. The standard curve obtained, easily fitted to a logit-log transformation, is useful over a concentration range of 6 to 400 ug/liter. Within-assay variability is 1.97%, between-assay variability 2.20%. The relation is linear when lactogen added is plotted against that accounted for analytically, the actual recovery being 100.26%.
Research Organization:
Loma Linda Univ., CA
OSTI ID:
6204404
Journal Information:
Clin. Chem.; (United States), Journal Name: Clin. Chem.; (United States) Vol. 24:10; ISSN CLCHA
Country of Publication:
United States
Language:
English