Development and characterization of a homologous radioimmunoassay for equine prolactin

Journal Article · Sun Apr 01 04:00:00 EST 1984 · Proc. Soc. Exp. Biol. Med.; (United States)

DOI:https://doi.org/10.3181/00379727-175-41829· OSTI ID:6974892

Roser, J F; Chang, Y S; Papkoff, H; Li, C H

A specific and sensitive homologous radioimmunoassay has been developed for equine prolactin, suitable for measuring prolactin concentrations in serum of horses. The sensitivity of the assay ranged from 0.4 to 0.6 ng/ml and the intra- and inter-assay coefficients of variation averaged 6.9 and 15.4%, respectively, for five doses of hormone. Cross-reactivity with other mammalian and nonmammalian prolactins and growth hormones was less than 20 and 0.3%, respectively. Cross-reactivity with equine growth hormone was less than 0.07%. Equine serum and pituitary extracts showed parallel dilution-response curves with equine prolactin. The percentage recovery of exogenous equine prolactin in serum was 89%. Preliminary analysis of several physiological samples (stallions, pregnant, and nonpregnant mares) yielded values from 0.6 to 12.0 ng/ml.

• Research Organization: Univ. of California, San Francisco
• OSTI ID: 6974892
• Journal Information: Proc. Soc. Exp. Biol. Med.; (United States), Journal Name: Proc. Soc. Exp. Biol. Med.; (United States) Vol. 175:4; ISSN PSEBA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BIOLOGICAL FUNCTIONS
BLOOD SERUM
FEMALES
FUNCTIONS
GONADOTROPINS
HORMONES
HORSES
ISOTOPE APPLICATIONS
LTH
MALES
MAMMALS
PEPTIDE HORMONES
PHYSIOLOGY
PITUITARY HORMONES
PREGNANCY
RADIOASSAY
RADIOIMMUNOASSAY
SENSITIVITY
STH
TRACER TECHNIQUES
VERTEBRATES