Changes in serum prolactin and corticosterone and pituitary prolactin and hypothalamic catecholamines in response to immobilization stress

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5385657

Palazzolo, D L; Quadri, S K

Effects of immobilization on serum prolactin (PRL) and corticosterone and on pituitary prolactin and hypothalamic catecholamines were determined in adult male Sprague-Dawley rats. The rats were immobilized for 0, 0.25, 0.5, 1, 3, or 6 hrs before decapitation at 1600 hrs. The hormones were determined by radioimmunoassays and catecholamines by high performance liquid chromatography. Immobilization for 15 min raised serum PRL from 13.1 +/- 2.6 ng/ml to 44.5 +/- 8.2 ng/ml. PRL levels returned to preimmobilization levels by 30 min and declined to 6.5 +/- 0.8 ng/ml by 6 hr (P < 0.05). Pituitary PRL concentrations decreased from 39.8 +/- 5.3 ug/pituitary at 0 hr to 27.2 +/- 2.2 ug/pituitary at 6 hr, indicating that continued stress causes a decrease in the synthesis and release of PRL. Serum corticosterone levels increased from 60.8 +/- 11.4 ng/ml at 0 hr to 198.5 +/- 42.7 ng/ml at 0.5 hr, then declined to 110.2 +/- 15.4 ng/ml by 6 hr indicating that, unlike PRL, high corticosterone levels are sustained during 6 hrs of stress. The hypothalamic concentrations (ng/mg tissue) of dopamine and norepinephrine declined from 0.72 +/- 0.13 and 3.2 + 0.2 at 0 hr to 0.28 +/- 0.08 and 1.2 +/- 0.3 respectively (P < 0.05) at 6 hr, most probably indicating an increased release of dopamine in the portal vessels which in turn led to the decrease in the synthesis and release of PRL.

• Research Organization: Kansas State Univ., Manhattan
• OSTI ID: 5385657
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AMINES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL STRESS
BODY
BRAIN
CARDIOTONICS
CARDIOVASCULAR AGENTS
CATECHOLAMINES
CENTRAL NERVOUS SYSTEM
CHROMATOGRAPHY
CORTICOSTEROIDS
CORTICOSTERONE
DOPAMINE
DRUGS
ENDOCRINE GLANDS
GLANDS
GLUCOCORTICOIDS
GONADOTROPINS
HORMONES
HYDROXY COMPOUNDS
HYPOTHALAMUS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
KETONES
LIQUID COLUMN CHROMATOGRAPHY
LTH
MAMMALS
NERVOUS SYSTEM
NEUROREGULATORS
NORADRENALINE
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHENOLS
PITUITARY GLAND
PITUITARY HORMONES
POLYPHENOLS
PREGNANES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RATS
RODENTS
SECRETION
SEPARATION PROCESSES
STEROID HORMONES
STEROIDS
SYMPATHOMIMETICS
TRACER TECHNIQUES
VERTEBRATES