Rat lymphoma cell bioassay for prolactin: observations on its use and comparison with radioimmunoassay

Journal Article · Mon Dec 27 04:00:00 EST 1982 · Life Sci.; (United States)

OSTI ID:6303054

Dawson, D M; Sensui, N; Haisenleder, D H; Gala, R R

The rat Nb/sub 2/ node lymphoma cell bioassay (BA) for prolactin (PRL) was validated for use in our laboratories. During the course of this validation we observed that rat prolactin (NIAMDD-RP-1) stimulated cell division by as much as 16.5 fold over the range of 0.04 to 40.0 ng/ml at the end of 72 hours of incubation. We also observed a dose related increase in the size of the lymphoma cells. Prolactin concentrations in rat plasma, serum, anterior pituitary (AP) homogenates and milk were measured by both radioimmunoassay (RIA) and BA. In individual BA's there was parallelism between samples and standard; but when several dilutions of the same plasma and pituitary homogenates were assayed repeatedly, higher PRL levels were consistently observed for the more concentrated samples. At low or moderate levels of plasma PRL there was excellent agreement between RIA and BA; however, at high levels plasma PRL bioactivity exceeded radioimmunoactivity by a small, but significant, amount. A comparison of pituitary PRL concentrations measured by RIA and BA were in good agreement when homogenization was done at pH 10.6. However, when homogenization was done at pH 7.6, slightly but significantly more PRL was extracted when assayed by BA than when assayed by RIA.

• Research Organization: Wayne State Univ. School of Medicine, Detroit, MI
• OSTI ID: 6303054
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 31:26; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMALS
BIOASSAY
COMPARATIVE EVALUATIONS
DISEASES
GONADOTROPINS
HORMONES
ISOTOPE APPLICATIONS
LTH
LYMPHOMAS
MAMMALS
MEASURING METHODS
NEOPLASMS
PEPTIDE HORMONES
PH VALUE
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
RATS
RESPONSE MODIFYING FACTORS
RODENTS
TRACER TECHNIQUES
VERTEBRATES