Relationship between bioassay and radioimmunoassay estimates of prolactin in rat serum

Journal Article · Wed Nov 01 04:00:00 EST 1978 · Endocrinology; (United States)

DOI:https://doi.org/10.1210/endo-103-5-1619· OSTI ID:6453743

Leung, F C; Russell, S M; Nicoll, C S

RIA and bioassay (BA) estimates of the concentration of PRL ((PRL)) in rat serum were compared using homologous assay systems. A rat mammary gland organ culture BA was developed which allowed objective and quantitative measurement of serum (PRL). The serum samples were tested at two doses against three doses of the RP-1 rat PRL standard and the responses were quantified by a histometric procedure. The index of precision of the BA ranged from 0.1 to 0.4 and the secretory response was not affected by physiological concentrations of rat GH, T/sub 3/, or by gonadal steroids. Serum samples taken from female rats during the estrous cycle, from suckled lactators, from rats bearing a PRL-secreting pituitary tumor, and from females that were treated with perphenazine were assayed. A highly significant correlation (r = 0.99) was obtained between the two assay methods with 21 samples. However, the regression coefficient was 4.20 +- 0.55. Thus, although the two assays are highly correlated for measuring serum (PRL), the RIA measures only about 25% of the hormone that is detected by the mammary gland organ culture BA.

• Research Organization: Univ. of California, Berkeley
• OSTI ID: 6453743
• Journal Information: Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 103:5; ISSN ENDOA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BIOASSAY
BLOOD SERUM
BODY
COMPARATIVE EVALUATIONS
DISEASES
ENDOCRINE GLANDS
GLANDS
GONADOTROPINS
HORMONES
ISOTOPE APPLICATIONS
LTH
MAMMALS
NEOPLASMS
ORGANS
PEPTIDE HORMONES
PITUITARY GLAND
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
RATS
RODENTS
TRACER TECHNIQUES
VERTEBRATES