Correlation and comparison of Nb/sub 2/ lymphoma cell bioassay with radioimmunoassay for human prolactin

Journal Article · Sat Dec 01 04:00:00 EST 1984 · Fert. Steril.; (United States)

OSTI ID:5943313

Subramanian, M G; Spirtos, N J; Moghissi, K S; Magyar, D M; Hayes, M F; Gala, R R

Serum samples from groups of men and women with normal and elevated prolactin (PRL) levels were assayed by radioimmunoassay (RIA) and by Nb/sub 2/ lymphoma cell bioassay (BA) for the presence of PRL. Because the Nb/sub 2/ lymphoma cells respond to both PRL and growth hormone, BA for PRL activity was carried out before and after neutralization of growth hormone in the serum samples. There were excellent correlations between RIA and BA both in euprolactinemic and hyperprolactinemic subjects. On an absolute basis, RIA and BA values were similar in the euprolactinemic group (6.6 +/- 0.8 versus 6.2 +/- 1.0), whereas in the hyperprolactinemic group, RIA values were significantly higher than the BA results. The two assay systems also appeared to correlate better in women who were hyperprolactinemic, with obvious menstrual cycle disturbances, than in hyperprolactinemic women without menstrual cycle disturbances.

• Research Organization: Wayne State Univ. School of Medicine, Detroit, MI
• OSTI ID: 5943313
• Journal Information: Fert. Steril.; (United States), Journal Name: Fert. Steril.; (United States) Vol. 42:6; ISSN FESTA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
BIOASSAY
BLOOD SERUM
COMPARATIVE EVALUATIONS
CORRELATIONS
DIAGNOSIS
DISEASES
GONADOTROPINS
HORMONES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LTH
LYMPHOMAS
NEOPLASMS
PATIENTS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
REPRODUCTIVE DISORDERS
TRACER TECHNIQUES
TUMOR CELLS