Chimaerism in lymph nodes of F/sub 1/ into irradiated parental recipient chimaeras rejecting skin allografts from the other parent. [x rays; mice]

Journal Article · Thu Jun 01 04:00:00 EDT 1978 · Transplantation; (United States)

OSTI ID:7085961

Suman, L; Silobrcic, V; Kastelan, A

Mice of the C57BL strain were irradiated with 800 R over the whole body. The next day they received i.v. a mixture of 50 x 10/sup 6/ spleen and bone marrow cells from (C57BL x CBA-T6T6)F/sub 1/ hybrid mice, and were challenged with CBA-T6T6 skin grafts later on. About 20% of the recipients rejected the CBA-T6T6 skin, whereas the others were completely tolerant for more than 200 days. By using the cytotoxic test, we found that both tolerant and nontolerant recipients were complete chimaeras, i.e., had only (C57BL x CBA-T6T6)F/sub 1/ cells in their lymph nodes. However, analysis of the same mice by the chromosome marker technique disclosed a proportion of host (C57BL) cells in lymph nodes of both tolerant and nontolerant chimaeras. The percentage of host metaphases in nontolerant chimaeras was significantly higher than that in tolerant chimaeras (P < 0.01). It is possible therefore that the CBA-T6T6 skin grafts were rejected by residual host (C57BL) cells rather than (C57BL x CBA-T6T6)F/sub 1/ cells reacting against skin-specific transplantation antigen(s) of the parental graft.

• Research Organization: Univ. of Zagreb
• OSTI ID: 7085961
• Journal Information: Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 25:6; ISSN TRPLA
• Country of Publication: United States
• Language: English

Related Subjects

551000 -- Physiological Systems
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE MARROW CELLS
CHIMERAS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
GRAFT-HOST REACTION
GRAFTS
IMMUNE REACTIONS
IMMUNOSUPPRESSION
INJECTION
INTAKE
INTRAVENOUS INJECTION
IONIZING RADIATIONS
IRRADIATION
LETHAL IRRADIATION
LYMPH NODES
LYMPHATIC SYSTEM
MAMMALS
MICE
MOSAICISM
ORGANS
RADIATION CHIMERAS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RODENTS
SKIN
SOMATIC CELLS
SPLEEN CELLS
TRANSPLANTS
VERTEBRATES
WHOLE-BODY IRRADIATION
X RADIATION