Common pathway for tumor cell uptake of gallium-67 and iron-59 via a transferrin receptor

Journal Article · Tue Jan 01 04:00:00 EST 1980 · J. Natl. Cancer Inst.; (United States)

OSTI ID:7079694

Larson, S M ^[1]; Rasey, J S; Allen, D R; Nelson, N J; Harp, G D; Williams, D L

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1. Veterans Administration Medical Center, Seattle, WA

We studied the tumor uptake of (/sup 67/Ga)citrate, (/sup 59/Fe)citrate, and /sup 125/I-labeled transferrin (TF) by the in vitro growth form of EMT-6, a sarcoma-like mammary tumor of BALB/c mice. In analyzing the binindg experiments, we developed a new mathematical model based on a formulation originally used to express the interaction of hormones with specific tissue receptors. The uptake of both carrier-free /sup 67/Ga and /sup 59/Fe by tumor cells was mediated by kinetically identical TF receptors. We also studied teric acid extracts of the stroma of EMT-6 tumors grown both in vivo and in vitro. Chromatography of these extracts on Sephacryl S-200 SF demonstrated that the cellular stroma contained specific TF-binding macromolecules. On the basis of these findings, we proposed the transferrin receptor hypothesis for the mechanism of /sup 67/Ga uptake by tumors. According to this view, a tumor-assisted TF receptor is the functional unit responsible for the affinity of gallum for certain neoplasms. This receptor was also active in the uptake of iron by tumors.

• OSTI ID: 7079694
• Journal Information: J. Natl. Cancer Inst.; (United States), Journal Name: J. Natl. Cancer Inst.; (United States) Vol. 64:1; ISSN JNCIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL PATHWAYS
CARBOHYDRATES
CARBOXYLIC ACID SALTS
CELL CULTURES
CHROMATOGRAPHY
CITRATES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
EXTRACTION CHROMATOGRAPHY
GALLIUM 67
GALLIUM ISOTOPES
GLUCOPROTEINS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IRON 59
IRON ISOTOPES
ISOTOPES
MAMMALS
MATHEMATICAL MODELS
MICE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
RADIONUCLIDE KINETICS
RECEPTORS
RODENTS
SACCHARIDES
SEPARATION PROCESSES
TUMOR CELLS
UPTAKE
VERTEBRATES